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Analyte detection

  • US 8,323,914 B2
  • Filed: 10/20/2011
  • Issued: 12/04/2012
  • Est. Priority Date: 09/18/1998
  • Status: Expired due to Fees
First Claim
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1. A method for detecting the presence of ethanol in a biological sample from a subject, comprising:

  • a) contacting an assay test comprising a reaction site comprising i) a chromagen, wherein said chromagen is potassium iodide, ii) an alcohol oxidase enzyme, and iii) a peroxidase enzyme with a biological sample from a subject under conditions such that said sample contacts said reaction site; and

    b) detecting the presence or absence of a visibly detectable signal in said reaction site, wherein said visibly detectable signal comprises a color change that occurs at a biological sample alcohol concentration at or above a threshold level and wherein no color change occurs below said threshold level.

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