Analyte detection

• US 8,323,914 B2
• Filed: 10/20/2011
• Issued: 12/04/2012
• Est. Priority Date: 09/18/1998
• Status: Expired due to Fees

• Current Assignee: N2itive1 Innovations
• Original Assignee: N2itive1 Innovations
• Inventors: Toranto, Anthony, Singer, Evan, Miller, Brett
• Primary Examiner(s): Cook, Lisa
• Application Number:
  US13/277,898
• Publication Number:
  US 20120034639A1
• Time in Patent Office: 411 Days
• Field of Search: None
• US Class Current: 435/7.21
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: G01N 33/98 involving alcohol, e.g. ethanol in breath
• CPC Class Codes: Y10T 436/204165 Ethanol

Abstract

The present invention relates to analyte detection test systems, including test systems for the oral detection of analytes in saliva. The present invention also provides compositions and methods for storing multiple assay tests and compositions and methods for measuring the concentration of analytes in a sample.

Claims

1. A method for detecting the presence of ethanol in a biological sample from a subject, comprising:

a) contacting an assay test comprising a reaction site comprising i) a chromagen, wherein said chromagen is potassium iodide, ii) an alcohol oxidase enzyme, and iii) a peroxidase enzyme with a biological sample from a subject under conditions such that said sample contacts said reaction site; and

b) detecting the presence or absence of a visibly detectable signal in said reaction site, wherein said visibly detectable signal comprises a color change that occurs at a biological sample alcohol concentration at or above a threshold level and wherein no color change occurs below said threshold level.

2. The method of claim 1, wherein said threshold level is equivalent to a blood alcohol concentration of 0.04%.

3. The method of claim 1, said assay test comprises a test strip.

4. The method of claim 3, wherein said test strip comprises an absorbent material, wherein said reaction site is located within said absorbent material.

5. The method of claim 1, wherein said color change is detectable by the human eye.

6. The method of claim 1, wherein said biological sample is a fluid.