Substrate for cell culture, producing method thereof and screening method for cell culture conditions utilizing the same

• US 8,323,973 B2
• Filed: 12/13/2004
• Issued: 12/04/2012
• Est. Priority Date: 12/16/2003
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Watanabe, Kohei, Miyazaki, Takeshi, Fujiyama, Tomoyo, Matsuda, Ryoichi, Kanome, Qsamu
• Primary Examiner(s): LAM, ANN Y
• Application Number:
  US10/582,099
• Publication Number:
  US 20070259394A1
• Time in Patent Office: 2,913 Days
• Field of Search: None
• US Class Current: 435/405
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/34 Internal compartments or partitions
• CPC Class Codes: C12N 2501/105 Insulin-like growth factors [IGF]
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/155 Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 2533/32 Polylysine, polyornithine
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12Q 1/045 Culture media therefor

Abstract

A cell culture substrate having at least one area for culturing a cell on a substrate, characterized in that the culturing area comprises an area releasably holding a biologically active substance having a biological activity to the cell and an area for immobilizing a biologically active substance having a biological activity to the cell.

Claims

1. A cell culture substrate having two or more culturing areas for culturing a cell on a substrate, wherein each of the two or more culturing areas contains a plurality of immobilization areas, the plurality of immobilization areas having different heights from the substrate and containing at least two biologically active substances having a biological activity to the cell, wherein the two or more culturing areas are exposed on a surface of the substrate, and wherein the two or more culturing areas contain different amounts of the at least two biologically active substances as a function of position.

2. The cell culture substrate according to claim 1, wherein the at least two biologically active substances are IGF-1 and bFGF.

3. The cell culture substrate according to claim 1, wherein the at least two biologically active substances are IGF-1, bFGF and BMP-2.

4. The cell culture substrate according to claim 1, wherein the immobilization areas are located at positions on a slope.

5. The cell culture substrate according to claim 4, wherein the slope is inclined with an angle of 25°

to 65°

relative to the substrate.