SPR apparatus with a high performance fluid delivery system

• US 8,325,346 B2
• Filed: 01/09/2012
• Issued: 12/04/2012
• Est. Priority Date: 12/18/2007
• Status: Active Grant

• Current Assignee: Suzhou Puxin Life Science & Technology Limited
• Original Assignee: Plexera, LLC
• Inventors: Kim, Gibum, Guan, Hannwen, Cong, Shuxin
• Primary Examiner(s): STAFIRA, MICHAEL PATRICK
• Application Number:
  US13/346,002
• Publication Number:
  US 20120154818A1
• Time in Patent Office: 330 Days
• Field of Search: 356445-448
• US Class Current: 356/445
• CPC Class Codes: G01N 21/01 Arrangements or apparatus for facilitating the optical investigation
• CPC Class Codes: G01N 21/11 Filling or emptying of cuvettes
• CPC Class Codes: G01N 21/55 Specular reflectivity
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Abstract

An apparatus may include a flow cell for a surface plasmon resonance test apparatus and a fluid delivery system operatively coupled to the flow cell and configured to deliver a plurality of fluids to the flow cell substantially continuously. The fluid delivery system may include two pumps for pumping the fluids.

Claims

1. A method for operating a surface plasmon resonance test apparatus comprising:

transmitting a first sequence of valve and pump control signals to a fluid delivery system coupled to a flow cell and a microarray;

priming the flow cell and microarray with a first solution;

receiving a plurality of images of the microarray;

analyzing the images of the microarray to determine if the microarray has been primed; and

if priming has occurred, automatically transmitting a second sequence including at least one valve control signal and at least one pump control signal to pump a second fluid through the flow cell and the microarray.

2. The method for operating the surface plasmon resonance test apparatus of claim 1, further comprising:

receiving at least one post-priming image of the microarray;

analyzing the post-priming image of the microarray to determine if the microarray remains primed; and

transmitting an alert message to a user if the microarray is not primed.

3. The method for operating the surface plasmon resonance test apparatus of claim 1, further comprising:

receiving at least one post-priming image of the microarray;

analyzing the post-priming image of the microarray to determine if the microarray remains primed; and

if the array is not primed, transmitting another second sequence including the at least one valve control signal and the at least one pump control signal to pump the first fluid through the flow cell and microarray.

4. The method for operating the surface plasmon resonance test apparatus of claim 1, wherein the surface plasmon resonance test apparatus includes an injection valve including a first port coupled to receive fluid pushed by a first pump and a second port coupled to receive fluid pushed by a second pump, wherein a sequence of valve and pump control signals switch the fluid delivery system between the first port and the second port.

5. The method for operating the surface plasmon resonance test apparatus of claim 4, wherein the surface plasmon resonance test apparatus includes a holding coil and a flow cell, wherein a first sequence of valve and pump control signals couple the fluid delivery system between the second pump and one or both the holding coil and the flow cell.

6. The method for operating the surface plasmon resonance test apparatus of claim 5, wherein a second sequence of valve and pump control signals couple the fluid delivery system between the second pump and the flow cell and between the first pump and the holding coil.

7. The method for operating the surface plasmon resonance test apparatus of claim 4, wherein a sequence of valve and pump control signals couple the fluid delivery system between the second pump to push fluid from the holding coil through the flow cell.

8. The method for operating the surface plasmon resonance test apparatus of claim 1, wherein the surface plasmon resonance test apparatus includes an injection valve including a first port coupled to receive fluid pushed by a first pump and a second port coupled to receive fluid pushed by a second pump, wherein a sequence of valve and pump control signals switch the fluid delivery system delivering to the holding coil via the injection valve using one or both the first pump and the second pump.

9. The method for operating the surface plasmon resonance test apparatus of claim 1, wherein the surface plasmon resonance test apparatus includes a selection valve and an injection valve including a first port coupled to receive fluid pushed by a first pump and a second port coupled to receive fluid pushed by a second pump, wherein a sequence of valve and pump control signals switch the fluid delivery system between the first pump and one or both a plurality of fluid sources and the injection valve.

10. The method for operating the surface plasmon resonance test apparatus of claim 9, wherein the plurality of fluid sources includes an auto sampler.

11. A method for operating a surface plasmon resonance test apparatus comprising:

transmitting a first sequence of valve and pump control signals to a fluid delivery system coupled to a flow cell and a microarray;

priming the flow cell and microarray with a first solution;

receiving a plurality of images of the microarray;

analyzing the images of the microarray to determine if the microarray has been primed; and

if priming has occurred, automatically transmitting a second sequence including at least one valve control signal and at least one pump control signal to pump a plurality of analytes sequentially through the flow cell and the microarray.

12. The method for operating the surface plasmon resonance test apparatus of claim 11, wherein the surface plasmon resonance test apparatus includes an injection valve including a first port coupled to receive fluid pushed by a first pump and a second port coupled to receive fluid pushed by a second pump, wherein a sequence of valve and pump control signals switch the fluid delivery system between the first port and the second port.

13. The method for operating the surface plasmon resonance test apparatus of claim 12, wherein the surface plasmon resonance test apparatus includes a holding coil and a flow cell, wherein the first sequence of valve and pump control signals couple the fluid delivery system between the second pump and one or both the holding coil and the flow cell.

14. The method for operating the surface plasmon resonance test apparatus of claim 13, wherein a second sequence of valve and pump control signals couple the fluid delivery system between the second pump and the flow cell and between the first pump and the holding coil.

15. The method for operating the surface plasmon resonance test apparatus of claim 12, wherein a sequence of valve and pump control signals couple the fluid delivery system between the second pump to push fluid from the holding coil through the flow cell.

16. The method for operating the surface plasmon resonance test apparatus of claim 11, wherein the surface plasmon resonance test apparatus includes an injection valve including a first port coupled to receive fluid pushed by a first pump and a second port coupled to receive fluid pushed by a second pump, wherein a sequence of valve and pump control signals switch the fluid delivery system delivering to the holding coil via the injection valve using one or both the first pump and the second pump.

17. The method for operating the surface plasmon resonance test apparatus of claim 11, wherein the surface plasmon resonance test apparatus includes a selection valve and an injection valve including a first port coupled to receive fluid pushed by a first pump and a second port coupled to receive fluid pushed by a second pump, wherein a sequence of valve and pump control signals switch the fluid delivery system between the first pump and one or both a plurality of fluid sources and the injection valve.

18. The method for operating the surface plasmon resonance test apparatus of claim 17, wherein the plurality of fluid sources includes an auto sampler.

19. The method for operating the surface plasmon resonance test apparatus of claim 11, wherein the surface plasmon resonance test apparatus includes a plurality of pumps, a holding coil, a flow cell and an injection valve including a plurality of ports coupled to receive fluid pushed by the plurality of pumps, a plurality of fluid couplings between the plurality of pumps and one or both the holding coil and the injection valve, wherein a sequence of valve and pump control signals switch the fluid delivery system between a first port and a second port such that a second analyte from the plurality of analytes does not contact a first analyte from the plurality of analytes in one or more of the injection valve, the plurality of fluid couplings and the holding cell.

20. A method for operating a surface plasmon resonance test apparatus comprising:

transmitting a first sequence of valve and pump control signals to a fluid delivery system coupled to a flow cell and a microarray;

priming the flow cell and microarray with a first solution;

at an initial time receiving a plurality of images of the microarray;

analyzing the images of the microarray to determine if the microarray has been primed;

if priming has occurred, automatically transmitting a second sequence including at least one valve control signal and at least one pump control signal to pump a plurality of analytes sequentially through the flow cell and the microarray;

at a later time receiving a plurality of images of the microarray;

analyzing the images of the microarray to determine if the microarray has degraded; and

if degradation has occurred, automatically transmitting a third sequence including at least one valve control signal and at least one pump control signal to pump a regeneration fluid through the flow cell and the microarray.