Devices and methods for sampling biological fluids

• US 8,329,114 B2
• Filed: 03/18/2009
• Issued: 12/11/2012
• Est. Priority Date: 03/18/2008
• Status: Active Grant

• Current Assignee: Strategic Operations Incorporated
• Original Assignee: Hemobotics LLC
• Inventors: Temple, John
• Primary Examiner(s): Gordon, Brian R
• Application Number:
  US12/406,815
• Publication Number:
  US 20090305407A1
• Time in Patent Office: 1,364 Days
• Field of Search: 422/501, 422504-505, 422/509, 422/512, 422/537, 422/540, 422/521, 435/307.1, 435/372
• US Class Current: 422/501
• CPC Class Codes: G01N 1/14 Suction devices, e.g. pumps; Ejector devices
• CPC Class Codes: G01N 1/18 with provision for splitting samples into portions G01N1/12, G01N1/14 take precedence; fraction-collection apparatus for chromatography B01D15/08

Abstract

Devices, instruments, systems and methods are provided in which a primary line that receives a biological fluid is selectively sampled by a plurality of collection chambers. Selective sampling occurs by selectively accessing the primary line and selective engagement of a sampling pump under control of a microprocessor. Further, the instrument housing reversibly houses a drive assembly and sample collection housing to permit the interchangeability of drive assemblies and collection housings and thus enhance sterility or reduction of cost.

Claims

1. A drive assembly with collection chambers for sampling biological fluids comprising:

a) a primary line having an inner cavity surrounded by a wall, said primary line comprising an an inlet end capable of receiving a biological fluid;

b) a fluid reservoir;

c) a waste container;

d) a plurality of sample collection chambers;

e) a peristaltic pump comprising a central rotor and a plurality of rotor housings, each capable of selective engagement with said central rotor, wherein each rotor housing comprises a sample collection line positioned along an inner perimeter and fluidly connected at one end to one of said plurality of collection chambers and at an opposing end to one of a plurality of needles, further wherein said engagement compresses said sample collection line against said central rotor and drives said needle into said inner cavity of said primary line; and

f) a cam assembly comprising a rotatable earn, positionable along said plurality of rotor housings, wherein rotation of said cam in a first position selectively engages one of said rotor housings with said central rotor and rotation in a second position releases said rotor housing from engagement with said central rotor.

2. The drive assembly according to claim 1, wherein said plurality of collection chambers each comprise a peltier cooling element.

3. The drive assembly according to claim 2, further comprising a reversibly attachable collection housing that houses said plurality of collection chambers, wherein each of said plurality of collection chambers each house a collection vial, tube or bag, further wherein said collection chambers are insulated.

4. The drive assembly according to claim 1, further comprising a threaded positioning rod complementary to a threaded aperture extending through said cam assembly, wherein rotation of said threaded positioning rod horizontally positions said cam assembly across and above said plurality of rotor housings.

5. The device according to claim 4, further comprising a rotating shaft slidably inserted through said cam, wherein rotation of said shaft rotates said cam into said first and second positions.

6. The device according to claim 4, further comprising a selecting valve for selecting communication between said primary line and said fluid reservoir, said primary line and said waste container, or said fluid reservoir and said waste container.

7. The device according to claim 4, further comprising a plurality of springs contacting said plurality of rotor housings, wherein at least one spring is compressed when said cam is in said first position and decompressed when in said second position.

8. A instrument for sampling a biological fluid comprising:

a) the drive assembly according to claim 1; and

b) an instrument housing, comprising;

i) a peristaltic pump motor that rotates said central rotor,

ii) a positioning motor for positioning said cam assembly,

iii) a cam motor for rotating said cam between said first position and said second position, and

iv) a microprocessor with memory and capable of connection to a power source, wherein said microprocessor instructs operation of said pump motor, positioning motor and cam motor.

9. The instrument according to claim 8, wherein said drive assembly is reversibly housed within said instrument housing and reversibly attachable to said peristaltic pump motor, positioning motor, and cam motor.

10. The instrument according to claim 9, wherein said instrument housing further comprises a peltier cooling element for cooling said collection chambers.

11. The instrument according to claim 10, further comprising a thermosistor.

12. The instrument according to claim 9, wherein,

a) said drive assembly further comprises:

i) a threaded positioning rod complementary to a threaded aperture extending through said cam assembly, wherein rotation of said threaded positioning rod horizontally positions said cam assembly across said plurality of rotor housings,

ii) a rotating shaft slidably inserted through said cam, wherein rotation of said shaft rotates said cam into said first and second positions,

iii) a plurality of springs associated with said plurality of rotor housings, wherein at least one spring is compressed when said cam is in said first position and decompressed when in said second position;

b) said positioning motor rotates said threaded positioning rod; and

c) said cam motor rotates said rotating shaft.

13. The instrument according to claim 12, wherein,

a) said drive assembly further comprises a selecting valve for selecting fluid communication between each of said primary line and said fluid reservoir, said primary line and said waste container and said fluid reservoir and said waste container;

and

b) said instrument housing further comprises a valve motor for operating said selecting valve.

14. A system for sampling a biological fluid comprising:

a) the instrument according to claim 13; and

b) an external control unit operably linked to said microprocessor, wherein said external control unit comprises a computer system comprising a software program, wherein said software program accepts entry of data corresponding to said a sample volume for collection into each of said collection chambers and a number of sample volumes for collection;

wherein said external control unit determines an order of sample collection and rinsing, and transfers said order as instructions to said microprocessor.

15. The device according to claim 1, wherein said plurality of needles remain recessed in said wall of said primary line when said rotor housings are not engaged.