Method and apparatus for determining one or more operating parameters for a microfluidic circuit

• US 8,329,118 B2
• Filed: 09/02/2004
• Issued: 12/11/2012
• Est. Priority Date: 09/02/2004
• Status: Active Grant

• Current Assignee: Honeywell International Inc.
• Original Assignee: Honeywell International Inc.
• Inventors: Padmanabhan, Aravind, Reutiman, Peter, Cabuz, Eugen I.
• Primary Examiner(s): Bullock, In Suk
• Assistant Examiner(s): Kingan, Timothy G
• Application Number:
  US10/932,662
• Publication Number:
  US 20060046300A1
• Time in Patent Office: 3,022 Days
• Field of Search: 422/50, 422/100, 422/68.1, 422/81, 422/82, 422/101, 422/102, 422/103, 436/43, 436/52, 436/53, 436/174, 436/177, 436/178, 436/180, 436/63, 700/266, 700/282
• US Class Current: 422/504
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2300/021 Identification, e.g. bar codes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G05D 7/0694 by action on throttling means or flow sources of very small size, e.g. microfluidics microvalves F16K99/0001; microstructural devices per se B81B
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
• CPC Class Codes: Y10T 137/0318 Processes
• CPC Class Codes: Y10T 137/0391 Affecting flow by the addition of material or energy
• CPC Class Codes: Y10T 137/0396 Involving pressure control
• CPC Class Codes: Y10T 137/8593 Systems
• CPC Class Codes: Y10T 137/85978 With pump
• CPC Class Codes: Y10T 436/12 Condition responsive control

Abstract

Methods for determining one or more operating parameters for a timing protocol of a microfluidic circuit are provided. In some embodiments, wet out times are measured for certain flow channels, and start times, flow rates and/or other parameters are calculated so that the various fluids in the microfluidic cartridge arrive at certain locations at a desired time and/or in a desired sequence. To help compensate for process variations, one or more fluidic process monitor components/structures may be fabricated along with the functional components/structures of a microfluidic cartridge. Test may be performed on the process monitor components/structures to help identify process variations in the particular microfluidic cartridge at hand. By using the process monitor data, the timing protocol for a particular microfluidic cartridge may be made more accurate.

Claims

1. A method for controlling the flow of fluid through a microfluidic cartridge, wherein the microfluidic cartridge includes two or more flow channels, wherein the two or more flow channels are fluidly connected at an intersection region, which is fluidly connected to a downstream flow channel, the method comprising the steps of:

causing flow of a first fluid in a first flow channel by applying a pressure to the first fluid from a first volume driven pressure source;

slowing or stopping the flow of the first fluid in the first flow channel when the first fluid reaches a predetermined location relative to the intersection region by reducing or eliminating the pressure applied from the first volume driven pressure source;

after the flow of the first fluid is slowed or stopped, causing a second fluid in the second flow channel to pass through the intersection region by applying a pressure to the second fluid from a second pressure source; and

with the second fluid passing through the intersection region, causing the flow of the first fluid in the first flow channel to flow through the intersection region by increasing the pressure from the first volume driven pressure source.

2. The method of claim 1 wherein the pressure provided by the first volume driven pressure source is controlled by one or more valves.

3. The method of claim 1 wherein the pressure provided by the first volume driven pressure source is controlled by one or more electrical signals.

4. The method of claim 1 wherein a second pressure source is used to cause the second fluid to flow through the second flow channel.

5. The method of claim 4 wherein the pressure provided by the second pressure source is controlled by one or more valves.

6. The method of claim 4 wherein the pressure provided by the second pressure source is controlled by one or more electrical signals.

7. The method of claim 4 wherein the second pressure source is a volume driven source.

8. The method of claim 1 wherein the predetermined location is upstream of the intersection region.

9. The method of claim 1 wherein the predetermined location is at or substantially at the intersection region.

10. The method of claim 1 wherein the predetermined location is downstream of the intersection region.

11. The method of claim 1 further comprising the step of slowing or stopping the flow of the second fluid in the second flow channel when the second fluid is upstream of the intersection region prior to causing the second fluid in the second flow channel to pass through the intersection region.

12. A method for controlling the flow of fluid through a microfluidic cartridge, wherein the microfluidic cartridge includes two or more flow channels, wherein the two or more flow channels are fluidly connected at an intersection region, which is fluidly connected to a downstream flow channel, and wherein the first flow channel extends along a path that extends between a first fixed location and the intersection region, the method comprising the steps of:

causing flow of a first fluid in the first flow channel at a first flow rate using a first pressure source, wherein the first pressure source applies a pressure to the first flow channel, and thus to the flowing first fluid, by delivering a pressure to the first fixed location of the first flow channel;

causing flow of a second fluid in a second flow channel at a second flow rate by applying a pressure to the second fluid from a second pressure source;

altering the flow rates of the first and/or second fluids in the first and second flow channels, respectively, when one of the first and second fluids reaches a predetermined location relative to the intersection region, such that one of the first and second fluids passes through the intersection region and the other of the first and second fluids remains upstream of the intersection region; and

causing the other of the first and second fluids to pass through the intersection region such that both the first and second fluids are then passing through the intersection region.

13. The method of claim 12 wherein the first and second flow rates are controlled by one or more valves controlled by one or more electrical signals.

14. The method of claim 12 wherein the step of altering the flow rates involves slowing or stopping the flow of the first fluid in the first flow channel when the first fluid reaches the predetermined location by reducing or eliminating the pressure applied from the first pressure source.

15. The method of claim 12 wherein the step of altering the flow rates involves increasing the first flow rate of the first fluid in the first flow channel when the fluid in the first flow channel reaches or substantially reaches the predetermined location.

16. A method for controlling the flow of fluid through a microfluidic cartridge, wherein the microfluidic cartridge includes two or more flow channels, wherein the two or more flow channels are fluidly connected at an intersection region, which is fluidly connected to a downstream flow channel, the method comprising the steps of:

causing flow of a first fluid in a first flow channel by applying a pressure to the first fluid from a first pressure source such that the first pressure source does not move along the first flow channel as the first fluid flows;

slowing or stopping the flow of the first fluid in the first flow channel when the first fluid reaches a predetermined location relative to the intersection region by reducing or eliminating the pressure applied from the first pressure source;

causing a second fluid in the second flow channel to pass through the intersection region by applying a pressure to the second fluid from a second pressure source; and

causing the flow of the first fluid in the first flow channel to flow through the intersection region by increasing the pressure from the first pressure source.

17. The method of claim 16 wherein the pressure provided by the first pressure source is controlled by one or more valves.

18. The method of claim 16 wherein the pressure provided by the first pressure source is controlled by one or more electrical signals.

19. The method of claim 16 wherein the pressure provided by the second pressure source is controlled by one or more valves.

20. The method of claim 16 wherein the pressure provided by the second pressure source is controlled by one or more electrical signals.

21. The method of claim 16 wherein the first pressure source is a volume driven source.

22. The method of claim 16 wherein the second pressure source is a volume driven source.

23. The method of claim 16 wherein the predetermined location is before the intersection region.

24. The method of claim 16 wherein the predetermined location is at or substantially at the intersection region.

25. The method of claim 16 wherein the predetermined location is past the intersection region.

26. The method of claim 16 further comprising the step of slowing or stopping the flow of the second fluid in the second flow channel when the second fluid is before the intersection region prior to causing the second fluid in the second flow channel to pass through the intersection region.

27. The method of claim 16 wherein the first pressure source causes the first fluid to flow in the first flow channel at a first flow rate, and the second pressure source causes the second fluid to flow in the second flow channel at a second flow rate.

28. The method of claim 27 wherein the first and second flow rates are controlled by one or more valves controlled by one or more electrical signals.