Microtome sectionable biopsy support for orienting tissue samples
First Claim
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1. A histologic tissue sample support device for orienting and retaining tissue samples during processing, embedding and sectioning in a microtome, comprising:
- a tissue cassette, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including first and second elongate channels for receiving at least one tissue sample, the first and second channels being separated from each other by separating structure, the separating structure connected with and extending upwardly from the bottom wall and including an anchoring element including a surface facing the bottom wall to anchor the separating structure in embedding material during a microtome sectioning process; and
tissue sample biasing structure configured to extend into the first and second channels to retain the tissue sample or samples against the bottom wall during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome.
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Abstract
Tissue orientation devices include a perforated tissue support with at least one perforated channel for receiving a tissue sample, and a plurality of tabs configured to extend along and into the channel to retain the tissue sample during processing and embedding. Tissue orientation devices include elongated legs coupled together for holding one or more biopsy tissue samples therebetween. Associated methods include using the cassettes and orientation devices to hold and orient tissue samples for processing, embedding and microtome sectioning.
156 Citations
21 Claims
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1. A histologic tissue sample support device for orienting and retaining tissue samples during processing, embedding and sectioning in a microtome, comprising:
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a tissue cassette, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including first and second elongate channels for receiving at least one tissue sample, the first and second channels being separated from each other by separating structure, the separating structure connected with and extending upwardly from the bottom wall and including an anchoring element including a surface facing the bottom wall to anchor the separating structure in embedding material during a microtome sectioning process; and tissue sample biasing structure configured to extend into the first and second channels to retain the tissue sample or samples against the bottom wall during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A histologic tissue sample support device, comprising:
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a frame; a tissue cassette releasably retained on the frame, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including first and second elongate channels for receiving at least one tissue sample, the first and second channels being separated from each other by separating structure, the separating structure connected with and extending upwardly from the bottom wall and including an anchoring element including a surface facing the bottom wall to anchor the separating structure in embedding material during a microtome sectioning process; and tissue sample biasing structure configured to extend into the first and second channels to retain the tissue sample or samples in place during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome. - View Dependent Claims (14, 15)
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16. A histologic tissue sample support device for orienting and retaining tissue samples during processing, embedding and sectioning in a microtome, comprising:
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a tissue cassette, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including first and second elongate channels for receiving at least one tissue sample, the first and second channels being separated from each other by separating structure, the separating structure connected with and extending upwardly from the bottom wall and configured to allow the tissue sample to extend from the first channel to the second channel; and tissue sample biasing structure configured to extend into the first and second channels to retain the tissue sample or samples against the bottom wall during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome.
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17. A histologic tissue sample support device, comprising:
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a frame; a tissue cassette releasably retained on the frame, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including first and second elongate channels for receiving at least one tissue sample, the first and second channels being separated from each other by separating structure, the separating structure connected with and extending upwardly from the bottom wall and configured to allow the tissue sample to extend from the first channel to the second channel; and tissue sample biasing structure configured to extend into the first and second channels to retain the tissue sample or samples in place during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome.
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18. A histologic tissue sample support device for orienting and retaining tissue samples during processing, embedding and sectioning in a microtome, comprising:
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a tissue cassette, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including an elongate channel for receiving at least one tissue sample, the channel including resilient fingers on opposite sides thereof for resiliently engaging and holding the tissue sample during processing and embedding; and tissue sample biasing structure configured to extend into the channel to retain the tissue sample or samples against the bottom wall during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome. - View Dependent Claims (19)
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20. A histologic tissue sample support device, comprising:
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a frame; a tissue cassette releasably retained on the frame, the tissue cassette including a perforated bottom wall and at least one perforated side wall for defining a tissue sample receiving space, the cassette formed of material which can be successfully sectioned in a microtome and is resistant to degradation from solvents and chemicals used to fix, process and stain tissue, the tissue cassette including an elongate channel for receiving at least one tissue sample, the channel including resilient fingers on opposite sides thereof for resiliently engaging and holding the tissue sample during processing and embedding; and tissue sample biasing structure configured to extend into the channel to retain the tissue sample or samples against the bottom wall during processing and embedding, the tissue sample biasing structure further being capable of successful sectioning in the microtome. - View Dependent Claims (21)
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Specification