Carbonic anhydrase bioreactor and process

• US 8,329,460 B2
• Filed: 04/07/2011
• Issued: 12/11/2012
• Est. Priority Date: 07/13/2001
• Status: Expired due to Term

• Current Assignee: Saipem SPA (Saipem)
• Original Assignee: CO2 Solutions Inc.
• Inventors: Parent, Carmen, Dutil, Frederic
• Primary Examiner(s): Bowers, Nathan
• Assistant Examiner(s): EDWARDS, LYDIA E
• Application Number:
  US13/081,804
• Publication Number:
  US 20110183397A1
• Time in Patent Office: 614 Days
• Field of Search: 435/299.1, 4322831-3094
• US Class Current: 435/299.1
• CPC Class Codes: B01D 2257/504 Carbon dioxide
• CPC Class Codes: B01D 53/84 Biological processes
• CPC Class Codes: B01D 53/85 with gas-solid contact
• CPC Class Codes: Y02A 50/20 Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
• CPC Class Codes: Y02C 20/40 of CO2
• CPC Class Codes: Y02E 50/30 Fuel from waste, e.g. synthetic alcohol or diesel

Abstract

A triphasic bioreactor for physico-chemically treating a gas is disclosed. The triphasic bioreactor comprises a reaction chamber with a liquid and biocatalysts in suspension in the liquid, for catalyzing a reaction between the gas and the liquid to obtain a treated gas and a solution containing a reaction product. A gas bubbling means is provided in the reaction chamber for bubbling the gas to be treated into the liquid thereby dissolving the gas into the liquid and increasing a pressure inside the reaction chamber. The bioreactor further comprises a liquid inlet in fluid communication with the reaction chamber for receiving the liquid and filling the reaction chamber, a liquid outlet in fluid communication with the reaction chamber for releasing the solution and a gas outlet in fluid communication with the reaction chamber to release the treated gas. The bioreactor further comprises a retention device to retain the biocatalysts in the reaction chamber. The invention also concerns a process using the triphasic bioreactor. The triphasic bioreactor may advantageously be used for removing carbonic dioxide from a CO₂-containing gas.

Claims

1. An enzymatic bioreactor for treatment of a CO₂-containing gas with carbonic anhydrase, the bioreactor comprising:

a reaction chamber for accommodating an absorption reaction of CO₂ in an aqueous solution containing the carbonic anhydrase, the carbonic anhydrase catalyzing the absorption reaction, thereby producing a CO₂-depleted gas stream and a liquid stream including hydrogen and bicarbonate ions, the carbonic anhydrase flowing through the reaction chamber;

a liquid inlet in fluid communication with the reaction chamber for feeding the aqueous solution containing carbonic anhydrase into the reaction chamber;

a gas inlet in fluid communication with the reaction chamber for feeding the CO₂-containing gas into the reaction chamber;

a liquid outlet in fluid communication with the reaction chamber for releasing the liquid stream, the liquid stream comprising the carbonic anhydrase;

a gas outlet in fluid communication with the reaction chamber to release the CO₂-depleted gas stream; and

a unit provided outside the reaction chamber for receiving the liquid stream from the liquid outlet and for removing the carbonic anhydrase from the liquid stream.

2. The carbonic anhydrase bioreactor according to claim 1, wherein the reaction chamber is configured and adapted to accommodate the carbonic anhydrase free in the aqueous solution.

3. The carbonic anhydrase bioreactor according to claim 1, wherein the carbonic anhydrase are provided in the form of a complex.

4. The carbonic anhydrase bioreactor according to claim 1, wherein the carbonic anhydrase are provided in a form that is of sub-micron size.

5. The carbonic anhydrase bioreactor according to claim 1, wherein the carbonic anhydrase are provided on or in supports.

6. The carbonic anhydrase bioreactor according to claim 5, wherein the carbonic anhydrase are provided on supports.

7. The carbonic anhydrase bioreactor according to claim 5, wherein the carbonic anhydrase are provided in supports.

8. The carbonic anhydrase bioreactor according to claim 1, wherein unit for removing the carbonic anhydrase from the liquid stream comprises a filter.

9. The carbonic anhydrase bioreactor according to claim 8, wherein the filter has pores with a smaller diameter than a diameter of the carbonic anhydrase, a support on or in which the carbonic anhydrase are supported or a complex of the carbonic anhydrase, for separating the carbonic anhydrase from the liquid stream.

10. The carbonic anhydrase bioreactor according to claim 9, wherein the filter comprises a membrane.

11. The carbonic anhydrase bioreactor according to claim 9, further comprising a pump in fluid communication with the liquid outlet and wherein the filter is provided downstream of the pump.

12. The carbonic anhydrase bioreactor according to claim 9, wherein the filter is an ultrafiltration filter.

13. The carbonic anhydrase bioreactor according to claim 9, wherein the filter is a microfiltration filter.

14. The carbonic anhydrase bioreactor according to claim 9, wherein the filter comprises a permeate outlet and a retentate outlet, the permeate outlet releasing a carbonic anhydrase depleted solution and the retentate outlet releasing a separated liquid containing the carbonic anhydrase.

15. The carbonic anhydrase bioreactor according to claim 14, comprising further treatment units for receiving the carbonic anhydrase depleted solution.

16. The carbonic anhydrase bioreactor according to claim 15, wherein the further treatment units are selected from decantation units and ion exchange units.

17. The carbonic anhydrase bioreactor according to claim 14, comprising a recycle line for recycling the separated liquid back into the reaction chamber.

18. The carbonic anhydrase bioreactor according to claim 1, wherein the carbonic anhydrase are provided linked in a network.

19. The process according to claim 1, wherein the removing the carbonic anhydrase from the liquid stream comprises filtering the carbonic anhydrase out of the liquid stream.

20. The process according to claim 19, wherein the filtering is performed by a membrane.

21. The process according to claim 19, further comprising pumping the liquid stream from the reaction chamber to the filtering.

22. The process according to claim 19, wherein the filtering is performed by ultrafiltration of the carbonic anhydrase.

23. The process according to claim 19, wherein the filtering is performed by microfiltration of the carbonic anhydrase.

24. The process according to claim 19, wherein the filtering comprises separating a carbonic anhydrase depleted solution and a separated liquid containing the carbonic anhydrase.

25. The process according to claim 24, comprising conveying the carbonic anhydrase depleted solution to further treatments.

26. The process according to claim 25, wherein the further treatments units are selected from decantation and ion exchange.

27. The process according to claim 24, wherein the separated liquid is recycled back into the reaction chamber.

28. An enzymatic process for treatment of a CO₂-containing gas with carbonic anhydrase, the process comprising:

feeding the CO₂-containing, gas into a reaction chamber;

feeding an aqueous solution containing carbonic anhydrase into the reaction chamber;

allowing an absorption reaction of CO₂ in the aqueous solution to occur within the reaction chamber, the carbonic anhydrase catalyzing the absorption reaction, thereby producing a CO₂-depleted gas stream and a liquid stream including hydrogen and bicarbonate ions, the carbonic anhydrase flowing through the reaction chamber;

releasing the CO₂-depleted gas stream and the liquid stream from the reaction chamber, the liquid stream comprising the carbonic anhydrase;

removing the carbonic anhydrase from the liquid stream outside of the reaction chamber.

29. The process according to claim 28, wherein the carbonic anhydrase are provided free in the aqueous solution.

30. The process according to claim 28, wherein the carbonic anhydrase are provided on or in supports.

31. The process according to claim 30, wherein the carbonic anhydrase are provided on supports.

32. The process according to claim 30, wherein the carbonic anhydrase are provided in supports.

33. The process according to claim 28, wherein the carbonic anhydrase are provided in the form of a complex.

34. The process according to claim 28, wherein the carbonic anhydrase are provided in a form that is of sub-micron size.

35. The process according to claim 28, wherein the carbonic anhydrase are provided linked in a network.