Targeted integration into the Zp15 locus
First Claim
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1. A method of integrating one or more exogenous nucleic acid sequences into the genome of a plant cell, the method comprising:
- making a double-stranded cleavage in the genome of the plant cell in a Zp15 locus using a zinc finger nuclease comprising a zinc finger binding domain that binds to a target site selected from the group shown in Table 1, thereby resulting in integration of a polynucleotide comprising the one or more exogenous sequences into the genome of the cell in the Zp15 locus.
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Abstract
Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into a plant Zp15 locus, for example, for expression of a polypeptide of interest.
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Citations
15 Claims
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1. A method of integrating one or more exogenous nucleic acid sequences into the genome of a plant cell, the method comprising:
making a double-stranded cleavage in the genome of the plant cell in a Zp15 locus using a zinc finger nuclease comprising a zinc finger binding domain that binds to a target site selected from the group shown in Table 1, thereby resulting in integration of a polynucleotide comprising the one or more exogenous sequences into the genome of the cell in the Zp15 locus. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
Specification