Process for comprehensively utilizing steam exploded radix puerariae and device therefor

• US 8,333,999 B2
• Filed: 07/27/2007
• Issued: 12/18/2012
• Est. Priority Date: 11/22/2006
• Status: Expired due to Fees

• Current Assignee: Institute of Process Engineering Chinese Academy of Sciences (Chinese Academy of Sciences), Hu Nan Qiangsheng Medicine Co., Ltd.
• Original Assignee: Institute of Process Engineering Chinese Academy of Sciences (Chinese Academy of Sciences), Hu Nan Qiangsheng Medicine Co., Ltd.
• Inventors: Chen, Hongzhang, Fu, Xiaoguo, Wang, Weidong
• Primary Examiner(s): TATE, CHRISTOPHER ROBIN
• Application Number:
  US12/447,895
• Publication Number:
  US 20100068778A1
• Time in Patent Office: 1,971 Days
• Field of Search: None
• US Class Current: 424/725
• CPC Class Codes: C12M 21/16 Solid state fermenters, e.g. for koji production
• CPC Class Codes: C12M 45/02 by mechanical forces; Stirring; Trituration; Comminuting crushing, pulverizing, disintegrating in general B02C
• CPC Class Codes: C12M 45/20 Heating; Cooling heating or cooling apparatus for laboratory uses B01L7/00
• CPC Class Codes: C12N 1/22 Processes using, or culture media containing, cellulose or hydrolysates thereof
• CPC Class Codes: C12P 17/06 containing a six-membered hetero ring, e.g. fluorescein
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The invention provides a process for co-producing ethanol and Radix Puerariae flavones and utilizing Radix Puerariae fibers from Radix Puerariae by way of clean solid state fermentation, and the device used therein. The process comprises the following steps: providing Radix Puerariae as raw material; pretreating the Radix Puerariae raw material by steam explosion; producing and separating ethanol by continuous coupled solid state fermentation; filtering off fermentation residues; and extracting and purifying Radix Puerariae flavones from the filtrate. The process achieves an effective and comprehensive utilization of Radix Puerariae.

Claims

1. A process for comprehensively utilizing Radix Puerariae, comprising:

providing Radix Puerariae as raw material;

pretreating the Radix Puerariae raw material by steam explosion;

producing and separating ethanol by continuous coupled solid state fermentation of the pretreated Radix Puerariae raw material;

filtering off a fermentation residue from the fermented Radix Puerariae raw material to obtain a filtrate; and

extracting and purifying Radix Puerariae flavones from the filtrate.

2. The process for comprehensively utilizing Radix Puerariae according to claim 1, further comprising:

separating the filtered fermentation residue into a fiber portion and a protein portion.

3. The process for comprehensively utilizing Radix Puerariae according to claim 1, wherein in said pretreating, the steam pressure is 0.5 to 1.0 MPa, and the steam explosion time is 2 to 4 minutes.

4. The process for comprehensively utilizing Radix Puerariae according to claim 1, wherein in said producing and separating, glucoamylase, (NH₄)₂SO₄, KH₂PO₄ and activated yeast are added for the fermentation of the pretreated Radix Puerariae raw material.

5. The process for comprehensively utilizing Radix Puerariae according to claim 4, wherein the added amount of said glucoamylase is 50˜

100 U/g of the pretreated Radix Puerariae raw material;

the added amount of said (NH₄)₂SO₄ is 0.05˜

0.15 g/100 g of the pretreated Radix Puerariae raw material;

the added amount of said KH₂PO₄ is 0.05˜

0.2 g/100 g of the pretreated Radix Puerariae raw material; and

the added amount of said activated yeast is 0.10˜

0.30 g yeast/100 g of the pretreated Radix Puerariae raw material.

6. The process according to claim 1, wherein in said extracting and purifying, model D-101, DS-401 or AB-8 macroporous adsorbing resin is used to remove impurities.

7. The process according to claim 1, wherein said continuous coupled solid state fermentation comprises:

providing a device comprising an airlock, a screw, a horizontal pot, a vertical pot;

a gas inlet and a gas outlet of said horizontal pot, a discharging airlock of said vertical pot, and a gas inlet and a gas outlet of said vertical pot;

providing the pretreated Radix Puerariae raw material to said horizontal pot through said airlock;

slowly propelling said screw inside said horizontal pot to move the provided pretreated Radix Puerariae raw material towards said vertical pot; and

continuously discharging the material present in said vertical pot through said airlock at the bottom of said vertical pot,

wherein a biochemical process selected from the group consisting of enzymolysis, fermentation and separation of the pretreated Radix Puerariae raw material is performed to completion.

8. The process according to claim 7, wherein the pretreated Radix Puerariae raw material is subjected to enzymolysis.

9. The process according to claim 7, wherein the pretreated Radix Puerariae raw material is subjected to fermentation.