Detection of nucleic acids from multiple types of human papillomaviruses
First Claim
Patent Images
1. A multiplex method of detecting the presence of type A1 and type C1 human papillomavirus (HPV) nucleic acids present in a biological sample, comprising the steps of:
- (a) treating a biological sample suspected of containing HPV type A1 or HPV type C1 to release intracellular components;
(b) separating an HPV nucleic acid away from a component of the biological sample;
(c) contacting the separated HPV nucleic acid with a combination of amplification oligonucleotides that amplify a HPV type A1 target sequences and a combination of amplification oligomers that amplify HPV type C1 target sequences in an E6/E7 target region sequence, wherein the combination of amplification oligomers configured to amplify HPV type A1 comprises amplification oligomers consisting essentially of SEQ ID NOS;
18, 19, 20, 21, 22, 23, 42 and 38(d) amplifying a HPV target sequence by using the amplification oligonucleotides and a nucleic acid polymerase in vitro to produce an HPV amplified product; and
(e) detecting the amplified product to indicate the presence in the sample of at least one of HPV types A1 or C1.
6 Assignments
0 Petitions
Accused Products
Abstract
Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed.
-
Citations
29 Claims
-
1. A multiplex method of detecting the presence of type A1 and type C1 human papillomavirus (HPV) nucleic acids present in a biological sample, comprising the steps of:
-
(a) treating a biological sample suspected of containing HPV type A1 or HPV type C1 to release intracellular components; (b) separating an HPV nucleic acid away from a component of the biological sample; (c) contacting the separated HPV nucleic acid with a combination of amplification oligonucleotides that amplify a HPV type A1 target sequences and a combination of amplification oligomers that amplify HPV type C1 target sequences in an E6/E7 target region sequence, wherein the combination of amplification oligomers configured to amplify HPV type A1 comprises amplification oligomers consisting essentially of SEQ ID NOS;
18, 19, 20, 21, 22, 23, 42 and 38(d) amplifying a HPV target sequence by using the amplification oligonucleotides and a nucleic acid polymerase in vitro to produce an HPV amplified product; and (e) detecting the amplified product to indicate the presence in the sample of at least one of HPV types A1 or C1. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
-
-
17. An amplification reaction mixture for use in a multiplex method of amplifying type A1 and type C1 human papillomavirus (HPV) nucleic acids present in a biological sample, wherein the reaction mixture contains (i) amplification oligomers configured to generate an amplification product from HPV type Al that consist essentially of SEQ ID NOS:
- 18, 19, 20, 21, 22, 23, 38 and 42 and (ii) amplification oligomers for generating an amplification product from HPV type C1.
- View Dependent Claims (18, 19, 20, 21)
-
22. A method of detecting the presence of type A1 and type C1 human papillomavirus (HPV) nucleic acids present in a biological sample, comprising the steps of:
-
(a) treating a biological sample suspected of containing HPV type A1 or HPV type C1 to release an HPV nucleic acid; (b) separating the HPV nucleic acid away from a component of the biological sample; (c) contacting the separated HPV nucleic acid with; (i) a combination of amplification oligonucleotides that amplify an HPV type A1 target sequence, the amplification oligonucleotides consisting essentially of SEQ ID NOS;
18, 19, 20, 21, 22, 23, 42 and 38; and(ii) a combination of amplification oligonucleotides that amplify an HPV type C1 target sequence, the amplification oligonucleotides consisting essentially of SEQ ID NOS;
28 or 30 and SEQ ID NO;
39;(d) amplifying a HPV target sequence by using the amplification oligonucleotides from step (c) and a nucleic acid polymerase to produce an HPV amplification product; and (e) detecting the amplification product to indicate the presence in the sample of at least one of HPV types A1 or C1. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29)
-
Specification