Methods and compositions for feeder-free pluripotent stem cell media containing human serum
First Claim
Patent Images
1. A tissue culture composition consisting of:
- a. a primate pluripotent stem cell;
b. a defined culture medium that supports proliferation of the primate pluripotent stem cell in an undifferentiated state; and
c. sufficient human serum to promote attachment of the primate pluripotent stem cell to a tissue culture vessel, wherein the tissue culture vessel is uncoated and free of matrix,wherein the composition does not comprise conditioned media and is essentially free of feeder cells.
3 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides compositions and methods for the culture and maintenance of pluripotent stem cells. More particularly, the present invention provides for compositions and methods for culturing, maintaining, growing and stabilizing primate pluripotent stem cells in a feeder-free defined media further comprising human serum, or a soluble attachment component of the human serum, for promoting cell attachment.
-
Citations
19 Claims
-
1. A tissue culture composition consisting of:
-
a. a primate pluripotent stem cell; b. a defined culture medium that supports proliferation of the primate pluripotent stem cell in an undifferentiated state; and c. sufficient human serum to promote attachment of the primate pluripotent stem cell to a tissue culture vessel, wherein the tissue culture vessel is uncoated and free of matrix, wherein the composition does not comprise conditioned media and is essentially free of feeder cells. - View Dependent Claims (2, 3, 4, 5, 6, 7)
-
-
8. A method of culturing a primate pluripotent stem cell comprising:
-
a. plating the primate pluripotent stem cell in a unconditioned and feeder-free combination consisting of; i. a defined culture media medium that supports proliferation of the primate pluripotent stem cell in a undifferentiated state; and ii. sufficient human serum to promote attachment of the primate pluripotent stem cell to a tissue culture vessel, wherein the tissue culture vessel is uncoated and is free of matrix, b. incubating the primate pluripotent stem cell plated in step a, thereby culturing the primate pluripotent stem cell. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
-
Specification