Polynucleotides comprising intercalating entities

• US 8,334,370 B1
• Filed: 10/05/1992
• Issued: 12/18/2012
• Est. Priority Date: 12/13/1985
• Status: Expired due to Fees

• Current Assignee: Enzo Biochem Incorporated
• Original Assignee: Enzo Biochem Incorporated
• Inventors: Rabbani, Elazar, Stavrianopoulos, Jannis
• Primary Examiner(s): MYERS, CARLA J
• Application Number:
  US07/956,566
• Time in Patent Office: 7,379 Days
• Field of Search: 536/22.1, 536/24.3, 536/25.32, 536/23.1, 435/6, 435/5, 935/5, 935/8, 935/77, 935/78, 436/164, 436/172, 436/800, 436/805
• US Class Current: 536/24.3
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 2537/101 Homogeneous assay format, e.g. one pot reaction
• CPC Class Codes: C12Q 2563/173 staining/intercalating agent, e.g. ethidium bromide
• CPC Class Codes: C12Q 2565/1015 labels being on the same oligonucleotide
• CPC Class Codes: C12Q 2565/107 Alteration in the property of hybridised versus free label oligonucleotides

Abstract

Provided are nucleic acid compositions modified with intercalating aromatic compounds attached directly or indirectly through a linker arm thereto. Modifications to the nucleotides or to the oligo- or polynucleotides involve the pentosyl moieties, the abasic moieties (without a base) and the base moieties. Useful property changes are effected and can be measured or detected when such modified nucleic acids compositions have hybridized specifically to target nucleic acids.

Claims

1. A single stranded polynucleotide comprising at least two intercalating entities which upon hybridization to a complementary target polynucleotide are capable of generating a detectable change in fluorescence emission of said entities, wherein each of said entities have the structure:

2. The polynucleotide of claim 1, wherein said entities are covalently attached to said nucleotides through an allylamine linker arm to the 5′

position of uracil nucleotides in said single stranded polynucleotide and said entities are separated from each other by about a stretch of 10 other nucleotides.

3. The polynucleotide of claim 1, wherein said entities are separated from each other by about a stretch of 10 other nucleotides.

4. The polynucleotide of claim 1, wherein said linker arms are allylamine linker arms.

5. The polynucleotide of claim 1, wherein said linker arms are attached to the base moiety of the nucleotides.