Methods of making enhanced, autologous fat grafts

• US 8,337,834 B2
• Filed: 03/07/2011
• Issued: 12/25/2012
• Est. Priority Date: 12/07/2001
• Status: Expired due to Fees

• Current Assignee: Lorem Vascular Pte. Ltd.
• Original Assignee: Cytori Therapeutics Incorporated
• Inventors: Fraser, John K., Hedrick, Marc H.
• Primary Examiner(s): LANKFORD JR, LEON B
• Application Number:
  US13/042,334
• Publication Number:
  US 20110158968A1
• Time in Patent Office: 659 Days
• Field of Search: None
• US Class Current: 424/93.7
• CPC Class Codes: A61B 10/0283 with vacuum aspiration, e.g. caused by retractable plunger or by connected syringe A61B10/025, A61B10/0266 take precedence
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 35/35 Fat tissue; Adipocytes; Stromal cells; Connective tissues adipose-derived stem cells A61K35/28; collagen A61K38/39
• CPC Class Codes: A61K 9/0019 Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: A61M 1/76 Handpieces specially for suction-irrigation A61M1/774, aspiration tips A61M1/84
• CPC Class Codes: A61M 2202/08 Lipoids
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 1/04 for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 13/12 of the kidneys
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 19/04 for non-specific disorders of the connective tissue
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: A61P 19/10 for osteoporosis
• CPC Class Codes: A61P 21/00 Drugs for disorders of the muscular or neuromuscular system
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 27/02 : Ophthalmic agents
• CPC Class Codes: A61P 3/00 : Drugs for disorders of the metabolism of the blood or the extracellular fluid A61P7/00
• CPC Class Codes: A61P 3/06 : Antihyperlipidemics
• CPC Class Codes: A61P 3/10 : for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 37/06 : Immunosuppressants, e.g. drugs for graft rejection
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/00 : Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/10 : for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: C12M 47/04 : Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: C12N 2506/1384 : from adipose-derived stem cells [ADSC], from adipose stromal stem cells
• CPC Class Codes: C12N 5/0667 : Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
• CPC Class Codes: C12N 5/069 : Vascular Endothelial cells

Abstract

Cells present in processed lipoaspirate tissue are used to treat patients. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Compositions that are administered to a patient include a mixture of adipose tissue and stem cells so that the composition has a higher concentration of stem cells than when the adipose tissue was removed from the patient.

Claims

1. A method of making an enhanced, autologous fat graft comprising extracting from a subject a first portion of adipose tissue to be used as a fat graft then supplementing said fat graft with a population of concentrated, disaggregated, adipose-derived stem cells and endothelial precursor cells in a buffered solution, wherein said stem cells and endothelial precursor cells are prepared by disaggregating and concentrating the stem cells and endothelial precursor cells from a second portion of extracted adipose tissue from said subject, so as to make said enhanced, autologous fat graft.

2. The method of claim 1, wherein said disaggregated adipose-derived stem cells and endothelial precursor cells are prepared by:

(a) disaggregating said second portion of extracted adipose tissue to generate a cell suspension; and

(b) separating a population of adipose-derived cells that comprises stem cells and endothelial precursor cells from the cell suspension by buoyant density sedimentation, filtration, or centrifugation.

3. The method of claim 2, wherein the second portion of extracted adipose tissue is disaggregated in (a) by enzymatic digestion or mechanical force.

4. The method of claim 2, further comprising reducing the presence of mature adipocytes in the cell suspension generated at step (a) prior to step (b).

5. The method of claim 2, wherein the population of adipose-derived cells that comprises stem cells and endothelial precursor cells in (b) is separated by centrifugation.

6. The method of claim 2, wherein the second portion of extracted adipose tissue is disaggregated in (a) by enzymatic digestion.

7. The method of claim 2, wherein said population of concentrated, disaggregated, adipose-derived stem cells and endothelial precursor cells is prepared in a device that operates in a closed system.

8. The method of claim 1, wherein said first portion of extracted adipose tissue is supplemented with said population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells in a device that operates in a closed system.

9. The method of claim 1, wherein said population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells is not cultured.

20. The method of claim 1, wherein the enhanced, autologous fat graft further comprises an additive.

10. A method of making an enhanced, autologous fat graft comprising:

(a) disaggregating a first portion of extracted adipose tissue from a subject with an enzyme or mechanical force to generate a cell suspension;

(b) concentrating the cells in said cell suspension by buoyant density sedimentation, filtration or centrifugation to obtain a population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells in a buffered solution; and

(c) supplementing a second portion of extracted adipose tissue to be used as a fat graft from said subject with the population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells in the buffered solution, so as to make said enhanced, autologous fat graft.

11. The method of claim 10, wherein the population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells in (b) is prepared by centrifugation.

12. The method of claim 10, wherein said population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells is prepared in a device that operates in a closed system.

13. The method of claim 10, wherein said second portion of extracted adipose tissue is supplemented with said population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells in a device that operates in a closed system.

14. The method of claim 10, further comprising reducing the presence of mature adipocytes in the cell suspension generated at step (a) prior to step (b).

15. The method of claim 10, wherein said population of concentrated, disaggregated adipose-derived stem cells and endothelial precursor cells is not cultured.

21. The method of claim 10, wherein the enhanced, autologous fat graft further comprises an additive.

16. A method of making an enhanced, autologous fat graft comprising extracting from a subject a first portion of adipose tissue to be used as a fat graft then supplementing said fat graft with a population of concentrated, disaggregated adipose-derived stem cells in a buffered solution, wherein said stem cells are prepared by disaggregating and concentrating the stem cells from a second portion of extracted adipose tissue from said subject, so as to make said enhanced, autologous fat graft.

17. The method of claim 16, wherein said population of concentrated, disaggregated adipose-derived stem cells is not cultured.

18. The method of claim 16, wherein said population of concentrated, disaggregated adipose-derived stem cells is prepared in a device that operates in a closed system.

19. The method of claim 16, wherein said second portion of extracted adipose tissue is supplemented with said population of concentrated, disaggregated stem cells in a device that operates in a closed system.

22. The method of claim 16, wherein the enhanced, autologous fat graft further comprises an additive.