Anti-hedgehog antibodies
First Claim
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1. A method for detecting human sonic hedgehog expression in tissue and/or determining if the polypeptide is overexpressed in said tissue comprising the steps of:
- (a) removing a tissue sample from a tumor in a patient afflicted with the tumor;
(b) contacting the tissue with an anti-human sonic hedgehog antibody comprising a heavy chain and a light chain, wherein the heavy chain comprises an HCHVR1 comprising the amino acid sequence of SEQ ID NO;
2, an HCHVR2 comprising the amino acid sequence of SEQ ID NO;
4 and an HCHVR3 comprising the amino acid sequence of SEQ ID NO;
6 and the light chain comprising an LCHVR1 comprising the amino acid sequence of SEQ ID NO;
10, an LCHVR2 comprising the amino acid sequence of SEQ ID NO;
12 and an LCHVR3 comprising the amino acid sequence of SEQ ID NO;
14, wherein said anti-hedgehog antibody binds to the polypeptide at an epitope within the region of amino acid residues 75-96,(c) measuring the extent of binding; and
(d) determining if human sonic hedgehog is overexpressed in said tissue, compared to normal tissue.
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Abstract
The invention relates to anti-hedgehog antibodies, their use in the detection of hedgehog expression in tissue, and to the use of such detection in the treatment of cancer.
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3 Claims
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1. A method for detecting human sonic hedgehog expression in tissue and/or determining if the polypeptide is overexpressed in said tissue comprising the steps of:
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(a) removing a tissue sample from a tumor in a patient afflicted with the tumor; (b) contacting the tissue with an anti-human sonic hedgehog antibody comprising a heavy chain and a light chain, wherein the heavy chain comprises an HCHVR1 comprising the amino acid sequence of SEQ ID NO;
2, an HCHVR2 comprising the amino acid sequence of SEQ ID NO;
4 and an HCHVR3 comprising the amino acid sequence of SEQ ID NO;
6 and the light chain comprising an LCHVR1 comprising the amino acid sequence of SEQ ID NO;
10, an LCHVR2 comprising the amino acid sequence of SEQ ID NO;
12 and an LCHVR3 comprising the amino acid sequence of SEQ ID NO;
14, wherein said anti-hedgehog antibody binds to the polypeptide at an epitope within the region of amino acid residues 75-96,(c) measuring the extent of binding; and (d) determining if human sonic hedgehog is overexpressed in said tissue, compared to normal tissue. - View Dependent Claims (2, 3)
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Specification