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Anti-hedgehog antibodies

  • US 8,338,112 B1
  • Filed: 08/13/2012
  • Issued: 12/25/2012
  • Est. Priority Date: 12/28/2007
  • Status: Active Grant
First Claim
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1. A method for detecting human sonic hedgehog expression in tissue and/or determining if the polypeptide is overexpressed in said tissue comprising the steps of:

  • (a) removing a tissue sample from a tumor in a patient afflicted with the tumor;

    (b) contacting the tissue with an anti-human sonic hedgehog antibody comprising a heavy chain and a light chain, wherein the heavy chain comprises an HCHVR1 comprising the amino acid sequence of SEQ ID NO;

    2, an HCHVR2 comprising the amino acid sequence of SEQ ID NO;

    4 and an HCHVR3 comprising the amino acid sequence of SEQ ID NO;

    6 and the light chain comprising an LCHVR1 comprising the amino acid sequence of SEQ ID NO;

    10, an LCHVR2 comprising the amino acid sequence of SEQ ID NO;

    12 and an LCHVR3 comprising the amino acid sequence of SEQ ID NO;

    14, wherein said anti-hedgehog antibody binds to the polypeptide at an epitope within the region of amino acid residues 75-96,(c) measuring the extent of binding; and

    (d) determining if human sonic hedgehog is overexpressed in said tissue, compared to normal tissue.

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