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Methods for making and using molecular switches involving circular permutation

  • US 8,338,138 B2
  • Filed: 01/28/2005
  • Issued: 12/25/2012
  • Est. Priority Date: 01/28/2004
  • Status: Active Grant
First Claim
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1. A method for assembling a modulatable fusion molecule polypeptide, comprising:

  • generating a circular permutation of an insertion nucleic acid sequence, wherein the insertion nucleic acid sequence encodes a polypeptide that recognizes an input signal; and

    inserting the insertion sequence into an acceptor nucleic acid sequence, wherein the acceptor sequence encodes a polypeptide that produces an output signal provided the output signal is not fluorescence, wherein the fused insertion and acceptor sequences encode a modulatable fusion polypeptide having the output signal functionally coupled to the input signal.

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