Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells

• US 8,338,170 B2
• Filed: 04/21/2008
• Issued: 12/25/2012
• Est. Priority Date: 04/21/2008
• Status: Active Grant

• Current Assignee: Viacyte Incorporated (Vertex Pharmaceuticals Incorporated)
• Original Assignee: Viacyte Incorporated (Vertex Pharmaceuticals Incorporated)
• Inventors: Kelly, Olivia, Bang, Anne
• Primary Examiner(s): Wilson, Michael C.
• Application Number:
  US12/107,020
• Publication Number:
  US 20090263896A1
• Time in Patent Office: 1,709 Days
• Field of Search: 435/325, 435/455
• US Class Current: 435/325
• CPC Class Codes: C12N 2501/50 Cell markers; Cell surface determinants
• CPC Class Codes: C12N 2501/599 with CD designations not provided for elsewhere
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 5/0676 Pancreatic cells
• CPC Class Codes: C12N 5/0678 Stem cells; Progenitor cells; Precursor cells
• CPC Class Codes: G01N 33/56966 Animal cells
• CPC Class Codes: G01N 33/68 involving proteins, peptides or amino acids involving lipoproteins G01N33/92

Abstract

The present disclosure relates to compositions and methods comprising cell surface markers for hES-derived cells, in particular, endoderm lineage cells including pancreatic endoderm-type cells, derived from hES cells.

Claims

1. A method of enriching for cells expressing pancreatic and duodenal homeobox gene 1 (PDX1) comprising:

a) exposing an in vitro cell population comprising PDX1 positive pancreatic cells to a ligand that binds CD142; and

b) selecting cells that express CD142 thereby enriching for cells expressing PDX1.

2. The method of claim 1, wherein said PDX1 positive cells express NKX6.1.

3. The method of claim 1, wherein the ligand comprises an antibody or binding fragment thereof.

4. The method of claim 1, wherein said ligand is associated with a detectable label.

5. The method of claim 1, wherein said ligand is associated with a magnetic particle.

6. The method of claim 1, wherein said cells are selected by Fluorescence Activated Cell Sorting (FACS).