Root evaluation
First Claim
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1. A method for non-destructive evaluation of roots of potted plants to identify plants possessing a trait of interest, comprising:
- (i) growing one or more plants in a container in which at least the bottom is substantially transparent, and wherein the container is charged with a particulate, non-transparent growing medium;
(ii) sequentially positioning the container with one or more plants for digital imaging of the roots of the plants by a camera below the bottom of the container; and
(iii) storing and analyzing information obtained from the plants to identify plants possessing a trait of interest.
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Abstract
A method is provided comprising (i) growing a plant in a substantially transparent container charged with a particulate, non-transparent growing medium; and (ii) evaluating plant roots through said transparent container by digital imaging. An apparatus for evaluating plant roots in a high throughput manner is also provided.
47 Citations
23 Claims
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1. A method for non-destructive evaluation of roots of potted plants to identify plants possessing a trait of interest, comprising:
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(i) growing one or more plants in a container in which at least the bottom is substantially transparent, and wherein the container is charged with a particulate, non-transparent growing medium; (ii) sequentially positioning the container with one or more plants for digital imaging of the roots of the plants by a camera below the bottom of the container; and (iii) storing and analyzing information obtained from the plants to identify plants possessing a trait of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. An apparatus for evaluating plant roots, comprising:
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(i) one or more containers in which at least the bottom is substantially transparent, wherein the one or more containers are charged with particulate, non-transparent growing medium for growing plants therein; (ii) a camera that can output images in a digital format; and (iii) a transporter for movement of the one or more containers or the camera; wherein the transporter positions the one or more containers or the camera for digital imagining by said camera below the bottom of the one or more containers. - View Dependent Claims (18, 19, 20, 21)
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22. A high-throughput method for non-destructive evaluation of roots of potted plants to identify plants possessing a trait of interest, comprising:
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(i) growing plants in a substantially transparent container charged with a particulate, non-transparent growing medium; (ii) providing each plant with a unique identifier; (iii) sequentially positioning each plant for digital imaging by a camera positioned below the bottom of the container of the roots through the transparent container and obtaining information concerning the roots of each plant associated with the plant'"'"'s unique identifier; (iv) providing the obtained information for each plant to an associated computer for identifying plants possessing a trait of interest.
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23. A method for non-destructive evaluation of roots of potted plants in a high throughput manner to identify plants possessing a trait of interest, comprising:
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(i) growing one or more plants in a container in which at least the bottom is substantially transparent and contains a pigment that absorbs all light wavelengths except between 500 and 600 nanometres, wherein the container is charged with a particulate, non-transparent growing medium comprising soil, and wherein each plant is provided with an unique identifier; (ii) sequentially positioning the container with one or more plants for digital imaging of the roots of the plants by a camera below the bottom of the container, wherein the camera is a line-scan camera that can output images in a digital format; and (iii) storing and analyzing information obtained from the plants to identify plants possessing a trait of interest.
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Specification