Electrode substrate, detection device equipped with electrode substrate, detection device kit and detection method using the kit

• US 8,343,333 B2
• Filed: 02/24/2006
• Issued: 01/01/2013
• Est. Priority Date: 03/07/2005
• Status: Expired due to Fees

• Current Assignee: Seiko Epson Corporation (Seiko Group)
• Original Assignee: Seiko Epson Corporation (Seiko Group)
• Inventors: Fukushima, Hitoshi, Takiguchi, Hiroshi
• Primary Examiner(s): Noguerola, Alex
• Assistant Examiner(s): Kaur, Gurpreet
• Application Number:
  US11/360,699
• Publication Number:
  US 20060196779A1
• Time in Patent Office: 2,503 Days
• Field of Search: 20440301-40315, 205/777.5, 205/778, 205/792, 600345-348, 422 50- 99, 422 681- 98, 435 4- 4052, 435/176, 435/177, 435/180, 435/181, 436 62- 71, 436500-548
• US Class Current: 205/792
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: C12Q 1/003 Functionalisation

Abstract

An electrode substrate including an electrode and a membrane provided on the electrode and having a thickness with which a carrier is exchanged between the electrode and the membrane.

Claims

1. An electrode substrate, comprising:

an electrode;

a membrane disposed on the electrode;

a mediator; and

a glucose oxidase,

the membrane having a monolayer including a chemical compound having a group represented by a following formula (1)

2. The electrode substrate according to claim 1,

a thickness of the membrane being 10-80 Å

, a carrier being exchanged between the electrode and the membrane.

3. The electrode substrate according to claim 1, wherein the electrode and the membrane are bonded through a sulfur atom or an oxygen atom.

4. A detection device, comprising:

the electrode substrate according to claim 1;

a counter electrode opposing the electrode substrate; and

a reference electrode.

5. The detection device according to claim 4, further comprising:

a detection circuit individually coupled to the electrode substrate, the counter electrode and the reference electrode.

6. A detection kit for detecting a subject material, comprising:

the detection device according to claim 4; and

a biomolecule or a mediator reacting with the subject material.

7. The detection kit according to claim 6, further comprising a buffer solution.

8. A method of detecting a subject material in a sample, comprising:

providing the detection kit according to claim 6; and

contacting the sample with the kit.

9. The method of detecting a subject material in a sample according to claim 8, further comprising:

measuring a current value in the presence of the subject material.

10. The electrode substrate according to claim 1,

the mediator including a ferocene or ferocene derivative.

11. The electrode substrate according to claim 1, further comprising:

a reaction coenzyme that is a pyroquinoline quinine or a nicotinamide adenine dinucleotide.

12. The electrode substrate according to claim 1, wherein a flavin adenine dinucleotide is at an active center of the glucose oxidase.