Biosensor

• US 8,343,427 B2
• Filed: 05/21/2009
• Issued: 01/01/2013
• Est. Priority Date: 05/26/2008
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Takahashi, Mie, Kawamata, Ryoko
• Primary Examiner(s): Sines, Brian J
• Application Number:
  US12/993,686
• Publication Number:
  US 20110070634A1
• Time in Patent Office: 1,321 Days
• Field of Search: 422/50, 422/68.1, 422/82.01
• US Class Current: 422/82.01
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

An inexpensive biosensor is provided that can realize an electrochemical analysis and perform measurement with high accuracy or convenience. The biosensor is an immunological sensor that enables measurement anywhere at anytime by anyone while keeping convenience. The biosensor includes: an electrode sensor unit 4 in which an electric conductive layer 2 is provided on an electrode-unit forming substrate 1 having an insulating property and an electrode portion 3 for detecting an electrochemical signal is formed on the electrode-unit forming substrate 1; and a chromatographic sensor unit 10 in which an extended layer 7 including a porous film for extending a specimen solution is provided on an extended-portion forming substrate 6, wherein the electrode sensor unit 4 and the chromatographic sensor unit 10 are stacked with the extended-portion forming substrate 6 interposed between the electrode sensor unit 4 and the chromatographic sensor unit 10.

Claims

1. A biosensor for qualitatively or quantitatively measuring a target analyte contained in a specimen solution, the biosensor comprising:

an electrode sensor unit including an electrode-unit forming substrate having an insulating property, an electric conductive layer provided on the electrode-unit forming substrate, and an electrode portion formed on the electrode-unit forming substrate for detecting an electrochemical signal;

a chromatographic sensor unit including an extended-portion forming substrate having an insulating property and an extended layer disposed on the extended-portion forming substrate for extending a specimen solution by a capillary flow;

a specimen solution supply portion for receiving the specimen solution, the specimen solution supply portion being provided at one of (a) a point facing the electrode portion and the extended layer and (b) a point communicating with the electrode portion and the extended layer; and

a reagent portion provided at at least one point of the electrode sensor unit, the extended layer of the chromatographic sensor unit, and the specimen solution supply portion,

wherein the electrode sensor unit and the chromatographic sensor unit are stacked, and

wherein the extended-portion forming substrate is interposed between the electric conductive layer of the electrode sensor unit and the extended layer of the chromatographic sensor unit.

2. The biosensor according to claim 1, further comprising:

reagent immobilizing portions on which a reagent specifically reacting with a target analyte is immobilized, the reagent immobilizing portions being provided partially on the extended layer; and

a labeled reagent retaining portion that is provided partially on one of the specimen solution supply portion and the extended layer and retains a labeled reagent soluble in contact with the specimen solution and specifically reacting with the target analyte,

wherein a target analyte component in the specimen solution is qualitatively or quantitatively measured by measuring a degree of reaction of the labeled reagent on the reagent immobilizing portions.

3. The biosensor according to claim 1, wherein the extended layer allows a blood component to be extended thereon without being filtered, and at least one response value in the extended layer and at least one response value in the electrode portion are measurable.

4. The biosensor according to claim 1, wherein the reagent portion containing a reagent specifically reacting with any substance in the specimen solution is provided partially on the electrode portion,

the reagent portion is capable of hydrating or dissolving in contact with the specimen solution, and

any substance in the specimen solution is qualitatively or quantitatively measured by measuring a degree of reaction in the reagent portion as a current response value.

5. The biosensor according to claim 1, wherein the reagent portion containing a reagent specifically reacting with a target analyte in the specimen solution is provided partially on the electrode portion and is capable of hydrating or dissolving in contact with the specimen solution,

the biosensor further comprising:

reagent immobilizing portions on which the reagent specifically reacting with the target analyte is immobilized, the reagent immobilizing portions being provided partially on the extended layer; and

a labeled reagent retaining portion that is provided partially on one of the specimen solution supply portion and the extended layer and retains a labeled reagent soluble in contact with the specimen solution and specifically reacting with the target analyte,

wherein any number of target analyte components in the specimen solution are qualitatively or quantitatively measured by measuring a degree of reaction in the reagent portion as a current response value and a degree of reaction of the labeled reagent in the reagent immobilizing portion.