Encapsulation of cells in biologic compatible scaffolds by coacervation of charged polymers
First Claim
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1. A method for encapsulating at least one cell in a biologically compatible scaffold comprising:
- (a) providing a first substrate coated with a first charged polymer;
(b) placing a scaffold comprising at least one cell embedded in a second charged polymer having an electrical charge opposite to that of the first charged polymer onto the first charged polymer on the first substrate;
(c) placing a second substrate coated with a third charged polymer to form a sandwich structure with the first substrate and the scaffold arranged therebetween, wherein the first polymer and the third polymer are of the same electrical charge; and
d) forming by complex coacervation of the second charged polymer with the first and third charged polymers a membrane formed of a polymer complex, wherein the polymer complex encapsulates the at least one cell therein.
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Abstract
This invention relates to a method for the encapsulation of cells in biologic compatible three dimensional scaffolds and the use of such cells encapsulated in a scaffold. The cells are embedded in a charged polymer that is complex coacervating with an oppositely charged polymer within biologic compatible scaffolds. The polymer complex embedding the cells is forming an ultra thin membrane on the surface of the three dimensional scaffold.
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Citations
17 Claims
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1. A method for encapsulating at least one cell in a biologically compatible scaffold comprising:
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(a) providing a first substrate coated with a first charged polymer; (b) placing a scaffold comprising at least one cell embedded in a second charged polymer having an electrical charge opposite to that of the first charged polymer onto the first charged polymer on the first substrate; (c) placing a second substrate coated with a third charged polymer to form a sandwich structure with the first substrate and the scaffold arranged therebetween, wherein the first polymer and the third polymer are of the same electrical charge; and d) forming by complex coacervation of the second charged polymer with the first and third charged polymers a membrane formed of a polymer complex, wherein the polymer complex encapsulates the at least one cell therein. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification