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Polymerase enzymes and reagents for enhanced nucleic acid sequencing

  • US 8,343,746 B2
  • Filed: 10/22/2007
  • Issued: 01/01/2013
  • Est. Priority Date: 10/23/2006
  • Status: Active Grant
First Claim
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1. A composition comprising:

  • a modified recombinant Φ

    29-type DNA polymerase, which modified recombinant polymerase comprises an amino acid sequence that is at least 90% identical to SEQ ID NO;

    1 and comprises an amino acid substitution at position 368 and an amino acid substitution at position 512, wherein numbering of positions is relative to SEQ ID NO;

    1, wherein the modified recombinant Φ

    29-type DNA polymerase exhibits polymerase activity; and

    a nucleotide analogue that is labeled on a phosphate group, wherein the base moiety of the analogue is selected from the group consisting of adenine, thymine, guanine, and cytidine.

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