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System for the production of aromatic molecules in Streptomyces

  • US 8,344,119 B2
  • Filed: 02/20/2008
  • Issued: 01/01/2013
  • Est. Priority Date: 02/21/2007
  • Status: Active Grant
First Claim
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1. A method for producing natural ferulic acid, coniferyl alcohol, vanillin or mixtures thereof, comprising:

  • a) culturing a bacterium belonging to the genus Streptomyces in a fermentation medium comprising eugenol, said bacterium comprising the nucleotide sequence of SEQ ID NO;

    1 or SEQ ID NO;

    8, or a nucleotide sequence having at least 90% sequence identity with the nucleotide sequence of SEQ ID NO;

    1 or SEQ ID NO;

    8, wherein the polypeptide encoded by the polynucleotide has vanillyl alcohol oxidase activity; and

    b) isolating the ferulic acid, coniferyl alcohol, vanillin or mixtures thereof produced in step (a).

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