Biosensor, thin film electrode forming method, quantification apparatus, and quantification method
First Claim
1. A method of manufacturing a biosensor for quantifying a substrate in a sample liquid comprising:
- providing an insulating support;
providing an electrically conductive layer that is made of a noble metal on a surface of the insulating support;
forming an electrode pattern comprising at least a working electrode and a counter electrode from the electrically conductive layer by dividing the electrically conductive layer using a laser to form the working electrode and the counter electrode;
providing a reagent layer formed on the electrode pattern;
providing a spacer layer on and covering part of the reagent layer, where the spacer layer defines a supply path for bringing the sample liquid into contact with the reagent layer and where the supply path formed by the spacer layer is narrower than the reagent layer; and
providing a cover on the spacer layer;
wherein an area of the counter electrode and an area of the working electrode are in the supply path, and the area of the counter electrode in the supply path is equal to or larger than the area of the working electrode in the supply path, andwherein part of the reagent layer is in the supply path.
5 Assignments
1 Petition
Accused Products
Abstract
A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
87 Citations
29 Claims
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1. A method of manufacturing a biosensor for quantifying a substrate in a sample liquid comprising:
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providing an insulating support; providing an electrically conductive layer that is made of a noble metal on a surface of the insulating support; forming an electrode pattern comprising at least a working electrode and a counter electrode from the electrically conductive layer by dividing the electrically conductive layer using a laser to form the working electrode and the counter electrode; providing a reagent layer formed on the electrode pattern; providing a spacer layer on and covering part of the reagent layer, where the spacer layer defines a supply path for bringing the sample liquid into contact with the reagent layer and where the supply path formed by the spacer layer is narrower than the reagent layer; and providing a cover on the spacer layer; wherein an area of the counter electrode and an area of the working electrode are in the supply path, and the area of the counter electrode in the supply path is equal to or larger than the area of the working electrode in the supply path, and wherein part of the reagent layer is in the supply path. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method of manufacturing a biosensor for quantifying a substrate in a sample liquid comprising:
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providing an insulating support; providing a working electrode formed from an electrically conductive layer, the layer is made of a noble metal and is provided on a surface of the insulating support; providing a reagent layer formed on the working electrode; providing a spacer layer on and covering part of the reagent layer, where the spacer layer defines a supply path for bringing the sample liquid into contact with the reagent layer and where the supply path formed by the spacer layer is narrower than the reagent layer; and providing a cover on the spacer layer, where the cover comprises a counter electrode formed from an electrically conductive layer, the layer is made of a noble metal and is provided on an inner surface of the cover; wherein an area of the counter electrode and an area of the working electrode are in the supply path, and the area of the counter electrode in the supply path is equal to or larger than the area of the working electrode in the supply path, and wherein part of the reagent layer is in the supply path. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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Specification