Detection of the presence or absence of a gas bubble by dynamic sensor response

• US 8,354,015 B2
• Filed: 12/01/2010
• Issued: 01/15/2013
• Est. Priority Date: 09/02/2004
• Status: Active Grant

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Kaltenbeck, Heinz, Grubler, Robert, Landschutzer, Egon
• Primary Examiner(s): Van, Luan
• Assistant Examiner(s): Kaur, Gurpreet
• Application Number:
  US12/957,513
• Publication Number:
  US 20110067483A1
• Time in Patent Office: 776 Days
• Field of Search: 204400-435, 205775-7945
• US Class Current: 205/775
• CPC Class Codes: G01N 2035/1018 Detecting inhomogeneities, e.g. foam, bubbles, clots
• CPC Class Codes: G01N 27/4163 checking the operation of, or calibrating, the measuring apparatus G01N27/3274, G01N27/4175 and G01N33/0006 take precedence
• CPC Class Codes: G01N 33/4925 measuring blood gas content, e.g. O2, CO2 , HCO3

Abstract

A method for detecting the presence or absence of a gas bubble in an aqueous liquid is provided comprising providing a sensor positioned within a measuring chamber, wherein the sensor is configured to determine the concentration of a gaseous component dissolved in a liquid, the sensor comprising a sensitive region; setting a gas partial pressure at the sensor, wherein the gas partial pressure differs from an expected value of the gas partial pressure of the gaseous component of a liquid to be measured; exposing the sensor to the liquid to be measured; resting the liquid until standstill is attained; recording a signal from the sensor as a function of time until the signal becomes constant; and detecting the presence or absence of a gas bubble from the variation of the signal over time. The gas bubble, if present, is in at least partial contact with the sensitive region of the sensor.

Claims

1. A method for detecting the presence or absence of a gas bubble in a liquid to be measured, where the gas bubble, if present, is in at least partial contact with a sensitive region of a sensor in a measuring chamber, the sensor for determining the concentration of a gaseous component dissolved in the liquid, the method comprising:

setting a gas partial pressure, which differs from an expected value of the gas partial pressure of the gaseous component in the liquid to be measured, at the sensor using a select one of;

an aqueous liquid, a gas, and a mixture of gases containing the gaseous component to be determined in suitable concentration;

exposing the sensor to the liquid to be measured;

waiting until the liquid to be measured attains standstill;

recording a signal from the sensor as a function of time until the signal becomes constant; and

inferring the presence or absence of a gas bubble from a variation of the signal over time.

2. The method of claim 1, wherein inferring the presence or absence of a gas bubble comprises:

measuring a signal curve value for at least two points in time during the response time of the sensor; and

comparing the measured signal curve values to known values obtained from a bubble-free sample.

3. The method of claim 1, wherein setting a gas partial pressure further comprises setting a gas partial pressure at the sensor before recording the signal, where the set gas partial pressure is greater than the gas partial pressure of the gaseous component in the liquid to be measured.

4. The method of claim 1, wherein setting the gas partial pressure comprises setting the gas partial pressure at the sensor using the aqueous liquid, where the aqueous liquid comprises at least one of:

a suitably tonometered calibrating fluid, a control fluid and a rinsing fluid.

5. The method of claim 1, further comprising employing the sensor as an O₂ sensor.

6. The method of claim 1, further comprising employing the sensor as a CO₂ sensor.

7. The method of claim 1, wherein the liquid to be measured comprises sample fluid.

8. The method of claim 7, wherein the sample fluid comprises a biological fluid.

9. The method of claim 8, wherein the biological fluid comprises blood.

10. The method of claim 1, wherein the liquid to be measured comprises a control fluid.

11. The method of claim 1, wherein the liquid to be measured comprises a calibrating fluid.