Fluorescent methods and materials for directed biomarker signal amplification
First Claim
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1. An assay method comprising:
- providing a sample that is suspected of containing a target biomolecule;
providing a multichromophore complex comprising a multichromophore covalently linked to a sensor biomolecule by a unique covalent bioconjugation site,wherein the multichromophore comprises the structure
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Abstract
Methods and compositions are provided that include a multichromophore and/or multichromophore complex for identifying a target biomolecule. A sensor biomolecule, for example, an antibody can be covalently linked to the multichromophore. Additionally, a signaling chromophore can be covalently linked to the multichromophore. The arrangement is such that the signaling chromophore is capable of receiving energy from the multichromophore upon excitation of the multichromophore. Since the sensor biomolecule is capable of interacting with the target biomolecule, the multichromophore and/or multichromophore complex can provide enhanced detection signals for a target biomolecule.
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Citations
35 Claims
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1. An assay method comprising:
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providing a sample that is suspected of containing a target biomolecule; providing a multichromophore complex comprising a multichromophore covalently linked to a sensor biomolecule by a unique covalent bioconjugation site, wherein the multichromophore comprises the structure - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. An assay method comprising:
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providing a sample that is suspected of containing a target biomolecule; providing a multichromophore complex comprising a multichromophore covalently linked to a sensor biomolecule by a unique covalent bioconjugation site, and a signaling chromophore covalently linked to the multichromophore complex, wherein the multichromophore comprises the structure - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. A kit for identifying a target biomolecule comprising:
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a multichromophore and a sensor biomolecule covalently linked to the multichromophore by a unique covalent bioconjugation site, wherein the sensor biomolecule is capable of specifically interacting with the target biomolecule, and wherein the multichromophore comprises the structure
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35. A kit for identifying a target biomolecule comprising:
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a multichromophore, a sensor biomolecule covalently linked to the multichromophore by a unique covalent bioconjugation site, and a signaling chromophore that is covalently bound to the multichromophore or is covalently bound to the sensor biomolecule, wherein the signaling chromophore is capable of receiving energy from the multichromophore upon excitation of the multichromophore and the sensor biomolecule is capable of specifically interacting with the target biomolecule, and wherein the multichromophore comprises the structure
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Specification