Electrochemical biosensor analysis system
First Claim
1. An electrochemical biosensor analysis system for analyzing a sample liquid, comprising:
- a biosensor comprising a carrier layer, an electrode structure located on the carrier layer and comprising at least two electrodes, a test field located on the carrier layer and covering the electrode structure, the test field comprising at least one layer of a material, the material configured to absorb the sample liquid, the test field further comprising a reagent system configured to react with the sample liquid and to produce a change of an electrical measurement variable, said measurement variable being characteristic for a desired analysis result and measurable by means of an analysis measurement employing the at least two electrodes, the biosensor further comprising a sample application surface on an upper side of the test field, the sample application surface facing generally away from the carrier layer and wherein the sample liquid penetrates through the sample application surface and into the test field via contact of the sample liquid onto the sample application surface from a location above the test field; and
an analysis instrument comprising an analytical measuring device configured for measuring the measurement variable of a sample liquid on the biosensor when the biosensor is positioned in a measurement position and electrically connected to the measuring device, the instrument further comprising an evaluation unit configured for determining the desired analysis data from measured values of the measurement variable;
wherein each of the at least two electrodes of the electrode structure comprises an array of conductor strips distributed on an effective electrode structure area, which area is wettable by the sample liquid, the distribution of the conductor strips of the electrode structure on the effective electrode structure area being generally homogeneous with respect to an AC resistance measurement performed on a liquid layer having a defined conductivity and layer thickness;
wherein the effective electrode structure area wettable by the sample liquid is congruently aligned with the sample application surface to which the sample liquid is applied, and the test field is configured for spreading a droplet of sample liquid applied to the sample application surface over at least a partial area of the sample application surface and for wetting a corresponding aligned area of the effective electrode structure area; and
the analysis instrument comprises an AC resistance measuring device configured for performing an area compensation measurement by measuring an AC resistance value between the at least two electrodes of the electrode structure, the measured AC resistance value being used, in the determination of the analysis data, as a measure of the extent that the area of the effective electrode structure area is wetted by the sample liquid.
3 Assignments
0 Petitions
Accused Products
Abstract
An electrochemical biosensor analysis system is provided for analyzing a sample liquid, comprising a biosensor having an electrode structure made of at least two electrodes, a test field, covering the electrode structure and capable of absorbing the sample liquid, and a defined sample application surface on the top side of the test field. The system also includes an analysis instrument comprising an evaluation unit for determining the desired analysis data. The analysis instrument comprises an AC resistance measuring device for measuring an AC resistance value between two electrodes of the electrode structure by means of an area compensation measurement. The measured AC resistance value is used, in the determination of the analysis data, as a measure of the partial area of the effective electrode structure area wetted by the sample liquid.
-
Citations
46 Claims
-
1. An electrochemical biosensor analysis system for analyzing a sample liquid, comprising:
-
a biosensor comprising a carrier layer, an electrode structure located on the carrier layer and comprising at least two electrodes, a test field located on the carrier layer and covering the electrode structure, the test field comprising at least one layer of a material, the material configured to absorb the sample liquid, the test field further comprising a reagent system configured to react with the sample liquid and to produce a change of an electrical measurement variable, said measurement variable being characteristic for a desired analysis result and measurable by means of an analysis measurement employing the at least two electrodes, the biosensor further comprising a sample application surface on an upper side of the test field, the sample application surface facing generally away from the carrier layer and wherein the sample liquid penetrates through the sample application surface and into the test field via contact of the sample liquid onto the sample application surface from a location above the test field; and an analysis instrument comprising an analytical measuring device configured for measuring the measurement variable of a sample liquid on the biosensor when the biosensor is positioned in a measurement position and electrically connected to the measuring device, the instrument further comprising an evaluation unit configured for determining the desired analysis data from measured values of the measurement variable; wherein each of the at least two electrodes of the electrode structure comprises an array of conductor strips distributed on an effective electrode structure area, which area is wettable by the sample liquid, the distribution of the conductor strips of the electrode structure on the effective electrode structure area being generally homogeneous with respect to an AC resistance measurement performed on a liquid layer having a defined conductivity and layer thickness; wherein the effective electrode structure area wettable by the sample liquid is congruently aligned with the sample application surface to which the sample liquid is applied, and the test field is configured for spreading a droplet of sample liquid applied to the sample application surface over at least a partial area of the sample application surface and for wetting a corresponding aligned area of the effective electrode structure area; and the analysis instrument comprises an AC resistance measuring device configured for performing an area compensation measurement by measuring an AC resistance value between the at least two electrodes of the electrode structure, the measured AC resistance value being used, in the determination of the analysis data, as a measure of the extent that the area of the effective electrode structure area is wetted by the sample liquid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
-
-
21. A biosensor for an analysis system, said biosensor comprising:
-
a carrier layer; an electrode structure located on the carrier layer, the electrode structure comprising at least two electrodes; a test field located on the carrier layer and generally covering the electrode structure, the test field comprising at least one layer of a material, the material configured to absorb a sample liquid applied to the biosensor and containing a reagent system configured to react with the sample liquid and to produce a change of an electrical measurement variable, said measurement variable being characteristic for a desired analysis result and measurable by means of an analysis measurement employing the at least two electrodes, the test field further comprising a sample application surface on an upper side of the test field and facing generally away from the carrier layer wherein the sample liquid penetrates through the sample application surface and into the test field via contact of the sample liquid onto the sample application surface from a location above the test field; wherein the at least two electrodes of the electrode structure are provided in an interdigitated array, each electrode comprising a primary conductor and a plurality of secondary conductors branching from the primary conductor and running transversely to the direction of the primary conductor, the secondary conductors of each electrode alternately interleaving with the secondary conductors of the other electrode to define the interdigitated array distributed on an effective electrode structure area, and wherein the effective electrode structure area wettable by the sample liquid is congruently aligned with the sample application surface to which the sample liquid is applied. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
-
-
35. A method for analyzing a sample liquid using a biosensor analysis system, comprising:
-
providing a biosensor comprising a carrier layer, an electrode structure located on the carrier layer and comprising at least two electrodes, and a test field located on the carrier layer and generally covering the electrode structure, the test field comprising at least one layer of a material, the material configured to absorb the sample liquid and containing a reagent system configured to react with the sample liquid and to produce a change of an electrical measurement variable, said measurement variable being characteristic for a desired analysis result and measurable by means of an analysis measurement employing the at least two electrodes, the test field further comprising a sample application surface on an upper side of the test field, the sample application surface facing generally away from the carrier layer, each of the at least two electrodes of the electrode structure comprising an array of conductor strips distributed on an effective electrode structure area which area is wettable by the sample liquid, the distribution of the conductor strips of the electrode structure on the effective electrode structure area being generally homogeneous with respect to an AC resistance measurement performed on a liquid layer having a defined conductivity and layer thickness, and wherein the effective electrode structure area wettable by the sample liquid is congruently aligned with the sample application surface to which the sample liquid is applied; providing an analysis instrument comprising an analytical measuring device configured for measuring the measurement variable while the biosensor is positioned in a measurement position and electrically connected to the measuring device, the analysis instrument further comprising an evaluation unit configured for determining the desired analysis data from the measured values of the measurement variable; contacting the sample application surface from a location above the test field with a droplet of the sample in such a manner that it diffuses over at least a partial area of the sample application surface and penetrates through the sample application surface and into the test field to thereby cause a corresponding partial area of the effective electrode structure area to be wetted; performing, within a time period during which the size of the wetted area does not significantly change, an analysis measurement in which the measurement variable is measured using the at least two electrodes; performing, within a time period during which the size of the wetted area does not significantly change, an area compensation measurement in which an AC resistance value is measured between the at least two electrodes of the electrode structure; and using the measured AC resistance value, when determining the analysis data, as a measure of the partial area of the effective electrode structure area wetted by the sample liquid. - View Dependent Claims (36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46)
-
Specification