System and method of deconvolving multiplexed fluorescence spectral signals generated by quantum dot optical coding technology
First Claim
1. A method of deconvolving a mixed fluorescence spectral signal into its component single-color fluorescence spectra emitted by contributing fluorophore types, wherein the method comprises:
- a) a database providing step of providing a spectral database that contains parameter sets, one for each of the component single-color fluorescence spectra;
b) a receiving step of receiving one or more encoded optical codes, each in the form of one said mixed fluorescence spectral signal emitted by a combination of the contributing fluorophore types; and
c) an analysis step, of analyzing each said mixed fluorescence spectral signal, comprising;
i) a peak estimation substep of using a sliding window technique, with reference to the parameter sets contained in the spectral database, to initially identify and estimate the number and location of local peak regions for each of the component single-color fluorescence spectra;
ii) a constraint setup substep of assigning allowable variation ranges for each of the parameter sets corresponding to one of the local peak regions for each said component single-color fluorescence spectra; and
iii) a deconvolution substep of deconvolving the mixed fluorescence spectral signal into a sum of parametric values, with reference to the parameter sets contained in the spectral database, so as to generate a list of its said component single-color fluorescence spectra;
whereby each encoded and received one of said optical codes, in the form of one said mixed fluorescence spectral signal, is decoded and identified by its said component single-color fluorescence spectra corresponding to its said contributing fluorophore types.
5 Assignments
0 Petitions
Accused Products
Abstract
A method deconvolves a multiplexed fluorescence spectral signal into its component single-color fluorescence spectra emitted by contributing fluorophore types. A spectral database contains a parameter set for each of the component spectra. Optical codes are received as the multiplexed signals. A sliding window technique estimates a number and location of local peaks for the component spectra. Allowable variation ranges are assigned for the corresponding parameter sets. Each multiplexed signal is deconvolved into a sum of parametric values to generate a list of its component spectra. Encoded and received optical codes, in the form of multiplexed signals, are decoded and identified by their component spectra and contributing fluorophore types. A system for deconvolving the multiplexed signals includes a spectral database containing the parameter sets, a detection element receiving the optical codes, and a signal processor encoded to analyze and deconvolve the multiplexed signals.
178 Citations
40 Claims
-
1. A method of deconvolving a mixed fluorescence spectral signal into its component single-color fluorescence spectra emitted by contributing fluorophore types, wherein the method comprises:
-
a) a database providing step of providing a spectral database that contains parameter sets, one for each of the component single-color fluorescence spectra; b) a receiving step of receiving one or more encoded optical codes, each in the form of one said mixed fluorescence spectral signal emitted by a combination of the contributing fluorophore types; and c) an analysis step, of analyzing each said mixed fluorescence spectral signal, comprising; i) a peak estimation substep of using a sliding window technique, with reference to the parameter sets contained in the spectral database, to initially identify and estimate the number and location of local peak regions for each of the component single-color fluorescence spectra; ii) a constraint setup substep of assigning allowable variation ranges for each of the parameter sets corresponding to one of the local peak regions for each said component single-color fluorescence spectra; and iii) a deconvolution substep of deconvolving the mixed fluorescence spectral signal into a sum of parametric values, with reference to the parameter sets contained in the spectral database, so as to generate a list of its said component single-color fluorescence spectra;
whereby each encoded and received one of said optical codes, in the form of one said mixed fluorescence spectral signal, is decoded and identified by its said component single-color fluorescence spectra corresponding to its said contributing fluorophore types. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
-
-
23. A system for deconvolving a mixed fluorescence spectral signal into its component single-color fluorescence spectra emitted by contributing fluorophore types, with the system being for use with one or more encoded optical codes, with each of the optical codes being in the form of one said mixed fluorescence spectral signal emitted by a combination of the contributing fluorophore types, wherein the system comprises:
-
a) a spectral database containing parameter sets, one for each of the component single-color fluorescence spectra; b) a detection element operatively receiving the optical codes; and c) a signal processor operative to analyze each said mixed fluorescence spectral signal, with the signal processor being encoded (i) to operatively execute a sliding window technique, with reference to the parameter sets contained in the spectral database, for initial identification and estimation of the number and location of local peak regions for each of the component single-color fluorescence spectra;
(ii) to assign allowable variation ranges for each of the parameter sets corresponding to one of the local peak regions for each said component single-color fluorescence spectra; and
(iii) to operatively deconvolve the mixed fluorescence spectral signal into a sum of parametric values, with reference to the parameter sets contained in the spectral database, so as to generate a list of its said component single-color fluorescence spectra;
whereby the system decodes and identifies each encoded and received one of said optical codes, in the form of one said mixed fluorescence spectral signal, by its said component single-color fluorescence spectra corresponding to its said contributing fluorophore types. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
-
Specification