Imaging method and device for carrying out said method

• US 8,361,440 B2
• Filed: 06/11/2003
• Issued: 01/29/2013
• Est. Priority Date: 06/11/2002
• Status: Expired due to Fees

• Current Assignee: Deutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
• Original Assignee: Deutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
• Inventors: Peter, Jrg
• Primary Examiner(s): CHAO, ELMER M
• Application Number:
  US10/517,637
• Publication Number:
  US 20050215873A1
• Time in Patent Office: 3,520 Days
• Field of Search: 600/407, 600/408, 600425-429, 600/414, 600/410, 600/420, 600/424, 600/431, 600/436, 424/9.1, 424/1.11, 424/9.6
• US Class Current: 424/9.1
• CPC Class Codes: A61B 2503/40 Animals
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 6/037 Emission tomography
• CPC Class Codes: A61B 6/508 for non-human patients
• CPC Class Codes: A61K 49/0013 Luminescence
• CPC Class Codes: A61K 49/0017 Fluorescence in vivo
• CPC Class Codes: A61K 51/1282 Devices used in vivo and carrying the radioactive therapeutic or diagnostic agent, therapeutic or in vivo diagnostic kits, stents

Abstract

The invention relates to an imaging method for simultaneously determining in vivo distributions of bioluminescent and/or fluorescent markers and radioactive markers at identical projection angles, the distribution of the bioluminescent and/or fluorescent markers being determined by separate detection of photons having a first average energy, which are emitted by the bioluminescent and/or fluorescent markers, by means of at least one first detector and the distribution of the radioactive markers being determined by simultaneous separate detection of photons having a second average energy, which are emitted by the radioactive markers, by means of at least one second detector. Furthermore, it also relates to an apparatus for carrying out the imaging method, containing at least one CCD camera (1, 2) as first detector, at least one single photon emission computer tomography (SPECT) detector (3) as second detector and a layer (5), which essentially reflects the photons of the bioluminescent and/or fluorescent markers and essentially transmits the photons of the radioactive markers.

Claims

1. An imaging method, comprising:

conducting an imaging procedure by simultaneously determining in vivo distributions of bioluminescent and/or fluorescent markers and in vivo distributions of radioactive markers of an object at identical projection angles;

wherein the distributions of the bioluminescent and/or fluorescent markers are determined by a first separate detection of photons having a first average energy, which are emitted by the bioluminescent and/or fluorescent markers, by at least one first detector;

wherein the distributions of the radioactive markers are determined by a second separate detection of photons having a second average energy, which are emitted by the radioactive markers, by at least one second detector;

wherein the photons of the bioluminescent and/or fluorescent markers having the first average energy and the photons of the radioactive markers having the second average energy are separated for the separate detection with the aid of a layer, the layer essentially reflecting the photons of the bioluminescent and/or fluorescent markers and transmitting the photons of the radioactive markers;

wherein the layer is used to reflect the photons of the bioluminescent and/or fluorescent markers in a first direction of the at least one first detector and for transmitting the photons of the radioactive markers in-a second direction of the at least one second detector, wherein the first and second directions are different from each other;

wherein the first separate detection and the second separate detection occur simultaneously;

wherein the at least one first detector, the at least one second detector, and the layer are fixedly arranged in a predetermined specific spatial arrangement relative to each other;

wherein the at least one first detector, the at least one second detector, and the layer are fixedly arranged as a rigid arrangement within a common housing;

wherein the object is placed within the housing during the imaging procedure; and

creating a first projection image from the first separate detection and a second projection image from the second separate detection, wherein the first and second projection images are of two-dimensional images of three-dimensional energy distributions of the bioluminescent and/or fluorescent markers and the radioactive markers of the object.

2. The imaging method as claimed in claim 1, wherein in the steps of determining the in vivo distributions, the bioluminescent and/or fluorescent markers comprise at least one marker from the group consisting of the markers of luciferase reporters, the marker molecules having emission wavelengths in the near infrared range (NIRF molecules) and the molecules of GFP (green fluorescent protein).

3. The imaging method as claimed in claim 1, wherein in the steps of determining the in vivo distributions, the radioactive markers comprise at least one marker from the group As-72, Br-75, Co-55, Cu-61, Cu-67, Ga-67, Gd-153, 1-123, 1-125, 1-131, In-111, Ru-97, Tl-201, Tc-99m and Xe-133.

4. The imaging method as claimed in claim 1, wherein the detection of the photons having the first average energy is carried out by at least one light detector camera providing the first detector and the detection of the photons having the second average energy is carried out by at least one single photon emission computer tomography (SPECT) detector providing the second detector comprising a collimator with at least one aperture.