×

Phosphotriester-modified oligonucleotide primers for nucleic acid amplification

  • US 8,361,753 B2
  • Filed: 05/17/2007
  • Issued: 01/29/2013
  • Est. Priority Date: 06/01/2006
  • Status: Active Grant
First Claim
Patent Images

1. A method of amplifying nucleic acids, said method comprising:

  • amplifying nucleic acid using a modified oligonucleotide primer,wherein said modified oligonucleotide primer comprises a modification group at one or two internucleotide linkages;

    wherein said modification groups are at the n, n-1, n-2, or n-3 positions, wherein n is the 3′

    terminal internucleotide linkage;

    wherein the presence of said modification group impairs nucleic acid polymerase extension of said modified oligonucleotide primer;

    wherein said modification group can be non-reversibly thermally dissociated; and

    wherein said modified oligonucleotide primer has structure I as follows

View all claims
  • 13 Assignments
Timeline View
Assignment View
    ×
    ×