Optical instrument comprising multi-notch beam splitter

• US 8,361,785 B2
• Filed: 01/26/2009
• Issued: 01/29/2013
• Est. Priority Date: 05/16/1998
• Status: Expired due to Fees

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Oldham, Mark F., Young, Eugene F.
• Primary Examiner(s): BOWERS, NATHAN ANDREW
• Application Number:
  US12/321,830
• Publication Number:
  US 20090141272A1
• Time in Patent Office: 1,464 Days
• Field of Search: 435/288.7, 435/808, 435/288.4, 435/303.1, 435/287.2, 250/459.1, 250/461.1, 250/461.2, 250/462.1, 250/483.1, 356/73, 378/42, 378/44, 378/45
• US Class Current: 435/288.7
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 2545/101 with an internal standard/control
• CPC Class Codes: G01N 2021/6419 Excitation at two or more wavelengths
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 2021/6463 Optics
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 2201/0245 with insertable-removable part
• CPC Class Codes: G01N 2201/0628 Organic LED [OLED]
• CPC Class Codes: G01N 2201/0635 Structured illumination, e.g. with grating
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/809 Incubators or racks or holders for culture plates or containers

Abstract

An instrument is provided that can monitor nucleic acid sequence amplification reactions, for example, PCR amplification of DNA and DNA fragments. The instrument includes a multi-notch filter disposed along one or both of an excitation beam path and an emission beam path. Methods are also provided for monitoring nucleic acid sequence amplifications using an instrument that includes a multi-notch filter disposed along a beam path.

Claims

1. An optical instrument comprising:

an excitation light source configured to provide a plurality of different excitation wavelength ranges;

a plurality of reaction regions comprising at least one respective sample including a fluorescent compound, the plurality of reaction regions simultaneously illuminated by the excitation light source so as to produce a plurality of emission beams, each emission beam comprising an emission wavelength range;

a beam splitter disposed to receive the emission beams from the plurality of reaction regions, the beam splitter disposed along an excitation beam path between the excitation light source and the plurality of reaction regions, and disposed along an emission beam path, wherein the beam splitter has an optical performance characterized in that light at wavelengths within each of the different excitation wavelength ranges is reflected and light at wavelengths within each of the emission wavelength ranges of the emission beams is transmitted; and

a detector to detect the emission beams of the emission wavelength ranges.

2. The instrument of claim 1, further comprising a Fresnel lens disposed along the excitation beam path.

3. The instrument of claim 1, further comprising a fold minor disposed along the excitation beam path.

4. The instrument of claim 1, further comprising a dichroic mirror disposed along the excitation beam.

5. The instrument of claim 1, wherein the beam splitter comprises a Rugate filter.

6. The instrument of claim 1, further comprising a processing device, operatively connected to the detector, the processing device configured to adjust exposure conditions of the detector to maintain a data signal from the detector within a predetermined operating range.

7. The instrument of claim 1, wherein the excitation light source comprises one or more lasers.

8. The instrument of claim 1, wherein the excitation light source comprises two irradiation sources.

9. The instrument of claim 8, wherein the excitation light source comprises at least one of a halogen lamp, a quantum dot, light emitting diode (“

LED”

), an organic LED, a phosphorescent organic LED, or a thin film electroluminescent device.

10. The instrument of claim 1, further comprising a field lens disposed to focus the emission beams onto the detector.

11. The instrument of claim 1, further comprising a field lens disposed to provide a telecentric optical system.

12. The instrument of claim 1, wherein the detector is a single array detector configured to receive emission beams from more than one of the plurality of reaction regions.

13. The instrument of claim 1, wherein the detector is a charge coupled device (CCD) or a charge injection device (CID), the detector configured to receive emission beams from more than one of the plurality of reaction regions.

14. The optical instrument of claim 1, further comprising an excitation filter disposed along the excitation beam path between the excitation light source and the beam splitter, the excitation filter comprising a multiple bandpass filter and configured to transmit light within the different excitation wavelength ranges.

15. The instrument of claim 1, wherein the excitation light source comprises a plurality of light emitting diodes having different wavelength ranges, the beam splitter being configured to reflect light from each of the light emitting diodes and transmit light from the emission beams.

16. The instrument of claim 1, wherein the fluorescent compound comprises a dye, marker, or label.

17. The instrument of claim 1, wherein the different excitation wavelength ranges are produced by different sources configured to be independently activated.

18. The instrument of claim 1, further comprising a plurality of detectors to detect the emission beams of the emission wavelength ranges.

19. A method comprising:

generating excitation beams from an excitation light source configured to provide a plurality of different excitation wavelength ranges;

directing the excitation beams to an excitation filter to produce filtered excitation beams;

directing the filtered excitation beams to a beam splitter;

simultaneously illuminating a plurality of reaction regions with light from the excitation light source, the plurality of reaction regions comprising at least one respective sample including a fluorescent compound;

causing the at least one respective sample from two or more reaction regions of the plurality of reaction regions to emit an emission beam comprising an emission wavelength range;

simultaneously directing the emission beams from the two or more reaction regions toward the beam splitter and toward a detector;

simultaneously detecting the two or more of the emission beams;

wherein the beam splitter reflects the different excitation wavelength ranges and transmits the emission wavelength ranges, or wherein the beam splitter transmits the different excitation wavelength ranges and reflects the emission wavelength ranges.

20. The method of claim 19, further comprising passing at least one of the excitation beams or the filtered excitation beams through a Fresnel lens.

21. The method of claim 19, further comprising passing the emission beams through a Fresnel lens.

22. The method of claim 19, further comprising reflecting at least one of the excitation beams or the filtered excitation beams with a fold mirror.

23. The method of claim 19, wherein each of the plurality of reaction regions is a well.

24. The method of claim 19, further comprising directing at least one of the excitation beams or the filtered excitation beams toward a dichroic minor.

25. The method of claim 19, further comprising adjusting exposure conditions of the detector to maintain a data signal within a predetermined operating range.

26. The method of claim 19, wherein the excitation beams are generated by one or more lasers.

27. The method of claim 19, wherein the excitation beams are generated by at least one of a halogen lamp, a quantum dot light emitting diode (“

LED”

), an organic LED, a phosphorescent organic LED, and a thin film electrolumiscent device.

28. The instrument of claim 19, further comprising simultaneously directing the emission beams from the two or more reaction regions toward a plurality of detectors.

29. A method comprising:

generating excitation beams from a light source configured to provide a plurality of different excitation wavelengths;

directing the excitation beams to an excitation filter to form filtered excitation beams comprising a selected excitation wavelength selected from two or more excitation wavelengths;

directing the filtered excitation beams to a beam splitter;

directing light from the light source to simultaneously illuminate a plurality of reaction regions comprising at least one respective sample comprising a plurality of different compounds;

for at least two of the compounds;

causing the samples of at least two reaction regions to emit emission beams comprising selected emission wavelength ranges;

simultaneously directing light from the at least two reaction regions toward the beam splitter and toward a detector;

transmitting the filtered excitation beams and reflecting the emission beams, or reflecting the filtered excitation beams and transmitting the emission beams;

detecting the emission beams with the detector.

30. The method of claim 29, wherein the light source provides a plurality of different excitation wavelength ranges and the filtered excitation beams comprises a selected excitation wavelength range selected from two or more excitation wavelength ranges.

31. The instrument of claim 29, further comprising simultaneously directing light from the at least two reaction regions toward a plurality of detectors.

32. An optical instrument configured to detect emission beams from a plurality of reaction regions including at least one respective sample having a fluorescent compound, the plurality of reaction regions configured to be simultaneously illuminated by an excitation light source, the instrument comprising:

an excitation light source comprising a plurality of light emitting diodes, the excitation light source configured to provide a plurality of different source wavelength ranges;

a detector configured to detect emission beams from a plurality of reaction regions, each emission beam comprising an emission wavelength range;

a beam splitter disposed along an emission beam path and configured to receive the emission beams from a plurality of reaction regions, the beam splitter disposed along an excitation beam path between the excitation light source and the plurality of reaction regions; and

an excitation filter disposed along the excitation beam path between the excitation light source and the beam splitter, the excitation filter comprising a multiple bandpass filter configured to transmit light within the plurality of different source wavelength ranges;

wherein the beam splitter has an optical performance characterized in that light at wavelengths within each of the source wavelength ranges is reflected and light at wavelengths within each of the emission wavelength ranges is transmitted.

33. The instrument of claim 32, further comprising a plurality of detectors to detect the emission beams from a plurality of reaction regions.

34. The instrument of claim 32, wherein the detector comprises an array detector, the beam splitter being configured to reflect the light from each of the light emitting diodes and transmit light from the emission beams.

35. The instrument of claim 32, wherein the fluorescent compound comprises a dye, marker, or label.

36. The instrument of claim 32, wherein the light emitting diodes are configured to be independently activated.

37. An optical instrument comprising:

an excitation light source comprising a plurality of light emitting diodes, the excitation light source configured to provide a plurality of different source wavelength ranges;

a region configured to include a plurality of reaction regions comprising at least one respective sample including a fluorescent compound, the excitation light source configured to simultaneously illuminate the plurality of reaction regions so as to produce a plurality of emission beams, each emission beam comprising an emission wavelength range;

a beam splitter disposed along an emission beam path and configured to receive the emission beams from the plurality of reaction regions, the beam splitter disposed along an excitation beam path between the excitation light source and the plurality of reaction regions; and

a detector configured to detect the emission beams;

wherein the beam splitter has an optical performance characterized in that light at wavelengths within each of the source wavelength ranges is reflected and light at wavelengths within each of the emission wavelength ranges is transmitted.

38. The optical instrument of claim 37, further comprising an excitation filter disposed along the excitation beam path between the excitation light source and the beam splitter, the excitation filter comprising a multiple bandpass filter and configured to transmit light within the plurality of different source wavelength ranges.

39. The optical instrument of claim 37, further comprising a plurality of detectors and a plurality of respective filters, each respective filter located along an optical path between one of the plurality of reaction regions and a detector of the plurality of detectors.

40. The instrument of claim 37, further comprising a plurality of detectors to detect the emission beams.

41. The instrument of claim 37, wherein the detector comprises an array detector, the beam splitter being configured to reflect the light from each of the light emitting diodes and transmit light from the emission beams.

42. The instrument of claim 37, wherein the fluorescent compound comprises a dye, marker, or label.

43. The instrument of claim 37, wherein the light emitting diodes are configured to be independently activated.

44. An optical instrument comprising:

an excitation light source comprising a plurality of light emitting diodes, the excitation light source configured to provide a plurality of different source wavelength ranges;

a block configured to hold a plurality of reaction regions comprising at least one respective sample including a fluorescent compound, the excitation light source configured to simultaneously illuminate the plurality of reaction regions so as to produce a plurality of emission beams, each emission beam comprising an emission wavelength range;

a beam splitter disposed along an emission beam path and configured to receive the emission beams from the plurality of reaction regions, the beam splitter disposed along an excitation beam path between the excitation light source and the plurality of reaction regions;

an excitation filter disposed along the excitation beam path between the excitation light source and the beam splitter, the excitation filter comprising a multiple bandpass filter and configured to transmit light within the plurality of different source wavelength ranges; and

a detector configured to detect the emission beams;

wherein the beam splitter has an optical performance characterized in that light at wavelengths within each of the source wavelength ranges is reflected and light at wavelengths within each of the emission wavelength ranges is transmitted.

45. The optical instrument of claim 44, further comprising a thermal controller for controlling a temperature of the block.

46. The optical instrument of claim 44, wherein the block is configured for heating and cooling by one or more of an electrical device, a liquid coolant device, or an air coolant device.

47. The instrument of claim 44, wherein the detector comprises an array detector, the beam splitter being configured to reflect the light from each of the light emitting diodes and transmit light from the emission beams.

48. The instrument of claim 44, wherein the fluorescent compound comprises a dye, marker, or label.

49. The instrument of claim 44, wherein the light emitting diodes are configured to be independently activated.

50. The instrument of claim 44, further comprising a plurality of detectors to detect the emission beams.

51. An optical instrument comprising:

an excitation light source configured to provide a plurality of different excitation wavelength ranges;

a plurality of reaction regions comprising at least one respective sample including a fluorescent compound, the plurality of reaction regions simultaneously illuminated by the excitation light source so as to produce a plurality of emission beams, each emission beam comprising an emission wavelength range;

a beam splitter disposed to receive the emission beams from the plurality of reaction regions, the beam splitter disposed along an excitation beam path between the excitation light source and the plurality of reaction regions, and disposed along an emission beam path, wherein the beam splitter has an optical performance characterized in that light at wavelengths within each of the different excitation wavelength ranges is transmitted and light at wavelengths within each of the emission wavelength ranges of the emission beams is reflected; and

a detector to detect the emission beams of the emission wavelength ranges.

52. An optical instrument configured to detect emission beams from a plurality of reaction regions including at least one respective sample having a fluorescent compound, the plurality of reaction regions configured to be simultaneously illuminated by an excitation light source, the instrument comprising:

an excitation light source comprising a plurality of light emitting diodes, the excitation light source configured to provide a plurality of different source wavelength ranges;

a detector configured to detect emission beams, each emission beam comprising an emission wavelength range;

a beam splitter disposed along an emission beam path and configured to receive the emission beams from a plurality of reaction regions, the beam splitter disposed along an excitation beam path between the excitation light source and the plurality of reaction regions; and

an excitation filter disposed along the excitation beam path between the excitation light source and the beam splitter, the excitation filter comprising a multiple bandpass filter configured to transmit light within the plurality of different source wavelength ranges;

wherein the beam splitter has an optical performance characterized in that light at wavelengths within each of the source wavelength ranges is transmitted and light at wavelengths within each of the emission wavelength ranges is reflected.

53. An optical instrument comprising:

an excitation light source comprising a plurality of light emitting diodes, the excitation light source configured to provide a plurality of different source wavelength ranges;

a region configured to include a plurality of reaction regions comprising at least one respective sample including a fluorescent compound, the excitation light source configured to simultaneously illuminate the plurality of reaction regions configured to be simultaneously illuminated by the excitation light source so as to produce a plurality of emission beams, each emission beam comprising an emission wavelength range;

a beam splitter disposed along an emission beam path and configured to receive the emission beams from the plurality of reaction regions, the beam splitter disposed along an excitation beam path between the excitation light source and the plurality of reaction regions; and

a detector configured to detect the emission beams;

wherein the beam splitter has an optical performance characterized in that light at wavelengths within each of the source wavelength ranges is transmitted and light at wavelengths within each of the emission wavelength ranges is reflected.