Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria

• US 8,362,228 B2
• Filed: 07/08/2011
• Issued: 01/29/2013
• Est. Priority Date: 04/19/2007
• Status: Expired due to Fees

• Current Assignee: Molecular Detection Inc.
• Original Assignee: Molecular Detection Inc.
• Inventors: Paitan, Yosef
• Primary Examiner(s): WOOLWINE, SAMUEL C
• Application Number:
  US13/178,807
• Publication Number:
  US 20110269140A1
• Time in Patent Office: 571 Days
• Field of Search: None
• US Class Current: 536/24.33
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: C12Q 2600/16 Primer sets for multiplex assays

Abstract

The present invention relates generally to detection of antibiotic-resistant bacteria in a sample. In particular, the invention provides methods, compositions and kits for detecting and analyzing methicillin-resistant Staphylococcus aureus (MRSA) and other methicillin-resistant bacteria in a sample.

Claims

1. A kit comprising:

(a) a first set of primers suitable for amplifying a polynucleotide sequence from a mecA gene;

(b) a second set of primers suitable for amplifying a polynucleotide sequence from a bridging region; and

(c) a third set of primers suitable for amplifying a polynucleotide sequence from a Staphylococcus aureus-specific gene, wherein said Staphylococcus aureus-specific gene is not orfX.

2. The kit of claim 1 which further comprises dNTPs.

3. The kit of claim 1 which further comprises a DNA polymerase.

4. The kit of claim 1 which further comprises a buffer.

5. The kit of claim 1, wherein said bridging region comprises the right extremity junction of SCCmec and orfX.