Methods for producing ZMWs with islands of functionality

• US 8,367,159 B2
• Filed: 12/07/2010
• Issued: 02/05/2013
• Est. Priority Date: 09/11/2009
• Status: Active Grant

• Current Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Original Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Inventors: Gray, Jeremy, Cicero, Ronald L., Grot, Annette, Popovich, Natasha, Dudek, Stephen
• Primary Examiner(s): Empie, Nathan
• Assistant Examiner(s): Jiang, Lisha
• Application Number:
  US12/962,249
• Publication Number:
  US 20110210094A1
• Time in Patent Office: 791 Days
• Field of Search: 427/282
• US Class Current: 427/264
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G02B 6/122 Basic optical elements, e.g. light-guiding paths

Abstract

The application relates to methods for producing islands of functionality within nanoscale apertures. Islands of functionality can be produced by growing an aperture constriction layer from the walls, functionalizing the exposed base of the aperture, then removing the aperture constriction layer. The aperture constriction layer can be produced, for example, by anodically growing an oxide layer onto a cladding through which the aperture extends. The islands of functionality can be used to bind a single molecule of interest, such as an enzyme within the nanoscale aperture.

Claims

1. A method for obtaining an island, of functionality at the bases of an array of zero mode waveguides comprising:

a) providing an electrochemical system comprising a working electrode, a counter electrode, and optionally a reference electrode, the working electrode in contact with an electrolyte solution;

b) providing a substrate having a lower transparent layer and an upper cladding layer, wherein the cladding layer comprises an array of apertures disposed through the cladding layer to the transparent layer, the apertures having side walls, wherein the cladding layer comprises the working electrode;

c) applying a voltage to the working electrode such that a layer of oxide is formed onto the side walls of the aperture;

d) attaching a functionalizing agent to exposed regions of the transparent layer within the apertures; and

e) dissolving the oxide layer from the walls of the aperture whereby islands of the functionalizing agent are formed within the apertures.

2. The method of claim 1 further comprising step (f) of attaching a single molecule of interest to the functionalizing agent on the transparent layer.

3. The method of claim 2 wherein step (f) is performed after step (e).

4. The method of claim 2 wherein step (f) is performed after step (d) and before step (e).

5. The method of claim 2 wherein the single molecule of interest comprises an enzyme or a nucleic acid.

6. The method of claim 2 wherein the percentage of apertures having only one single molecule of interest is greater than 37%.

7. The method of claim 1 further comprising performing steps (a), (b), and (c) again after step (e) whereby a second oxide layer is formed to produce an array of apertures having islands of the functionalizing agent and oxide layers on the walls.

8. The method of claim 1 wherein step (e) of dissolving the oxide layer is carried out so as to dissolve some of the oxide layer and leave some of the oxide layer undissolved to produce an array of apertures having islands of the functionalizing agent and oxide layers on the walls.

9. The method of claim 1 wherein the electrolyte solution comprises a metal salt that forms a gel layer on the surface of the cladding layer.

10. The method of claim 9 wherein the metal salt comprises a salt of antimony, molybdenum, silica, or tungsten.

11. A method for producing an array of nanostructures comprising:

a) providing a substrate having a top surface, the top surface having an aperture layer, the aperture layer having a plurality of apertures extending through the aperture layer to the substrate, wherein the substrate is exposed by the apertures, each of the apertures having one or more cross-sectional dimension;

b) oxidizing the substrate whereby an oxide layer is formed on the aperture layer, whereby a cross sectional dimension of the apertures is brought to 50 nm or smaller, leaving a portion of the substrate exposed;

c) treating the substrate with a functionalizing agent whereby the functionalizing agent becomes attached to the exposed portions of the substrate;

d) exposing the substrate to nanostructures to attach the nanostructures to the functionalizing agent attached to the substrate; and

e) dissolving the oxide layer whereby islands of the functionalizing agent are formed within the apertures.

12. The method of claim 11 wherein the nanostructures comprise nanoparticles.

13. A method for producing an island of functionalizing agent in an array of zero mode waveguides comprising:

a) providing a substrate having on its surface a cladding layer, wherein the cladding layer comprises an array of apertures disposed through the cladding layer to the substrate, the apertures having side walls;

b) selectively growing an aperture constriction layer on the cladding layer such that the aperture constriction layer extends in from the side walls of the aperture to reduce the cross-sectional dimensions of the aperture;

c) attaching a functionalizing agent to exposed regions of the substrate within the apertures; and

d) removing the aperture constriction layer whereby an array of apertures, each having an island of the functionalizing agent is produced.

14. The method of claim 13 wherein the aperture constriction layer comprises a polymer.

15. The method of claim 13 wherein the aperture constriction layer comprises a metal oxide.

16. The method of claim 13 wherein the aperture constriction layer comprises a metal.

17. The method of claim 13 wherein the step of selectively growing an aperture constriction layer comprises growing a polymer from the cladding with a polymerization reaction extending from the surface of the cladding.

18. The method of claim 13 wherein the step of selectively growing an aperture constriction layer comprises growing an oxide onto the cladding by connecting the cladding to a voltage source under conditions such that controlled oxidation of the cladding occurs.

19. The method of claim 13 wherein the step of selectively growing an aperture constriction layer comprises electrodepositing a material onto the cladding by connecting the cladding to a voltage source and providing the current required for electrodeposition.

20. The method of claim 13 wherein the apertures after step (d) have a cylindrical profile and have a diameter between about 70 nm and 300 nm.

21. The method of claim 13 wherein the islands have a diameter between about 5 nm and about 50 nm.

22. The method of claim 13 wherein the constriction layer comprises a gel layer on the surface of the cladding layer comprising one or more metal salts.

23. The method of claim 22 wherein the metal salt comprises a salt of antimony, molybdenum, silica, or tungsten.