Method for synthesizing DNA
First Claim
1. A DNA synthesis reaction composition comprising:
- 1) a DNA polymerase having 3′
→
5′
exonuclease activity;
2) water-soluble acidic macromolecular substances selected from the group consisting of κ
-carrageenan, dermatan sulfate, hyaluronic acid, polyvinyl sulfates, polystyrene sulfates, sodium polyglutamate, polyacrylic acids, and water-soluble salts thereof; and
3) components necessary for DNA synthesis using DNA polymerase.
1 Assignment
0 Petitions
Accused Products
Abstract
A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.
74 Citations
11 Claims
-
1. A DNA synthesis reaction composition comprising:
-
1) a DNA polymerase having 3′
→
5′
exonuclease activity;2) water-soluble acidic macromolecular substances selected from the group consisting of κ
-carrageenan, dermatan sulfate, hyaluronic acid, polyvinyl sulfates, polystyrene sulfates, sodium polyglutamate, polyacrylic acids, and water-soluble salts thereof; and3) components necessary for DNA synthesis using DNA polymerase. - View Dependent Claims (2, 3, 4)
-
-
5. A kit for use in in vitro DNA synthesis, comprising:
-
1) DNA polymerases having 3′
→
5′
exonuclease activity;2) water-soluble acidic macromolecular substances selected from the group consisting of κ
-carrageenan, dermatan sulfate, hyaluronic acid, polyvinyl sulfates, polystyrene sulfates, sodium polyglutamate, polyacrylic acids, and water-soluble salts thereof; and3) components necessary for DNA synthesis using DNA polymerase. - View Dependent Claims (6, 7)
-
-
8. A DNA synthesis reaction composition comprising:
-
1) a DNA polymerase having 3′
→
5′
exonuclease activity;2) a water-soluble acidic macromolecular substance; and 3) components necessary for DNA synthesis using DNA polymerase, wherein the water-soluble acidic macromolecular substance is sodium polyacrylate.
-
-
9. A DNA synthesis reaction composition comprising:
-
1) a DNA polymerase having 3′
→
5′
exonuclease activity;2) a water-soluble acidic macromolecular substance; and 3) components necessary for DNA synthesis using DNA polymerase, wherein the water-soluble acidic macromolecular substance is sodium polyglutamate.
-
-
10. A kit for use in in vitro DNA synthesis, comprising:
-
1) DNA polymerases having 3′
→
5′
exonuclease activity;2) a water-soluble acidic macromolecular substance; and 3) components necessary for DNA synthesis using DNA polymerase, wherein the water-soluble acidic macromolecular substance is sodium polyacrylate.
-
-
11. A kit for use in in vitro DNA synthesis, comprising:
-
1) DNA polymerases having 3′
→
5′
exonuclease activity;2) a water-soluble acidic macromolecular substance; and 3) components necessary for DNA synthesis using DNA polymerase, wherein the water-soluble acidic macromolecular substance is sodium polyglutamate.
-
Specification