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Programmable oligonucleotide synthesis

  • US 8,367,335 B2
  • Filed: 04/06/2012
  • Issued: 02/05/2013
  • Est. Priority Date: 08/23/2006
  • Status: Active Grant
First Claim
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1. A method for isolating an individual nucleic acid molecule having a desired correct sequence from a mixture of nucleic acids, comprising:

  • (a) monoclonizing a mixture of nucleic acids on a solid reaction support;

    (b) parallel sequencing the monoclonized nucleic acids of step (a),(c) localizing a nucleic acid molecule with the desired correct sequence in the nucleic acids of step (b),(d) isolating the nucleic acid molecule from step (c), and(e) optionally further processing of the isolated nucleic acid molecule obtained in step (d).

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