Process for producing sugars and ethanol using corn stillage

• US 8,367,378 B2
• Filed: 10/03/2008
• Issued: 02/05/2013
• Est. Priority Date: 10/03/2007
• Status: Expired due to Fees

• Current Assignee: The Board of Trustees of Michigan State University (Michigan State University)
• Original Assignee: The Board of Trustees of Michigan State University (Michigan State University)
• Inventors: Balan, Venkatesh, Chundawat, Shishir, Sousa, Leonardo, Dale, Bruce
• Primary Examiner(s): Saidha, Tekchand
• Assistant Examiner(s): Meah, Younus
• Application Number:
  US12/286,913
• Publication Number:
  US 20090093027A1
• Time in Patent Office: 1,586 Days
• Field of Search: None
• US Class Current: 435/99
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A process for producing sugars from lignocellulosic materials such as corn stover by the addition of corn stillage as a carbon source is disclosed. The sugars are formed by treating the combination of the corn stillage and the lignocellulosic materials with hydrolytic enzymes. The sugars can be fermented to ethanol, and the process improves ethanol production economies with an increased sugar yield from the lignocellulosic materials.

Claims

1. A process for producing sugars from a lignocellulosic material and corn comprising:

pretreating the lignocellulosic material with an ammonia treatment process to produce a pretreated lignocellulosic material, wherein ammonia in the ammonia pretreatment process is present in a concentration ranging from about 0.2 to about 4 grams ammonia per gram of the lignocellulosic material;

separating corn grain stillage into a substantially liquid fraction comprising about 0.05 wt. % to about 50 wt. % crude protein and a substantially solid fraction;

adding the substantially liquid fraction to the pretreated lignocellulosic material; and

hydrolyzing complex carbohydrates in the pretreated lignocellulosic material in an aqueous medium with one or more hydrolyzing enzymes to form the sugars, wherein the substantially liquid fraction comprises some or all of the aqueous medium.

2. The process of claim 1, wherein the corn grain stillage comprises whole corn grain stillage with solids.

3. The process of claim 1, wherein the corn grain stillage comprises thin corn grain stillage.

4. The process of claim 1, wherein the lignocellulosic material comprises corn stover.

5. The process of claim 1, wherein the lignocellulosic material comprises one or more of miscanthus, rice straw, poplar, and bagasse.

6. The process of claim 1, wherein the hydrolyzing is performed in an aqueous medium which contains the pretreated lignocellulosic material, the corn grain stillage, and the one or more hydrolyzing enzymes, wherein the pretreated lignocellulosic material comprises glucan and the aqueous medium has a glucan loading as a component of the pretreated lignocellulosic material ranging from about 0.1 wt. % to about 20 wt. %.

7. The process of claim 1, wherein the hydrolyzing is performed in an aqueous medium which contains the pretreated lignocellulosic material, the corn grain stillage, and the one or more hydrolyzing enzymes, wherein the aqueous medium comprises about 0.5 wt. % to about 99 wt. % corn grain stillage relative to the aqueous medium.

23. The method of claim 1 wherein the corn grain stillage comprises about 0.1% corn grain protein.

24. The method of claim 1 wherein the corn grain stillage comprises about 0.5% to about 10% corn grain protein.

25. The method of claim 1 wherein the corn grain stillage comprises about 1% corn grain protein.

26. The method of claim 1 wherein the corn grain stillage comprises about 1% to about 4% corn grain protein.

27. The method of claim 26 wherein the corn grain stillage comprises about 2% corn grain protein.

28. The method of claim 1 wherein the ammonia in the ammonia pretreatment process is present in a concentration ranging from about 0.2 to about 2 grams ammonia per gram of the lignocellulosic material.

29. The method of claim 28 wherein the ammonia in the ammonia pretreatment process is present in a concentration ranging from about 0.5 to about 1 grams ammonia per gram of the lignocellulosic material.

8. A process for producing sugars from a lignocellulosic material and corn comprising:

pretreating the lignocellulosic material with an ammonia treatment process to produce a pretreated lignocellulosic material, wherein ammonia in the ammonia pretreatment process is present in a concentration ranging from about 0.2 to about 4 grams ammonia per gram of the lignocellulosic material;

separating corn grain stillage into a protein-containing thin stillage component and a wet grain component;

adding the protein-containing thin stillage component to the pretreated lignocellulosic material; and

hydrolyzing complex carbohydrates in the pretreated lignocellulosic material with one or more hydrolyzing enzymes to form the sugars, wherein the hydrolyzing is performed in an aqueous medium which contains the pretreated lignocellulosic material, the protein-containing thin stillage component, and the hydrolyzing enzymes, wherein the aqueous medium comprises about 0.02 wt. % to about 5 wt. % crude corn protein as a component of the protein-containing thin stillage component relative to the aqueous medium.

9. The process of claim 8, wherein the one or more hydrolyzing enzymes comprise one or more cellulases and, optionally, one or more hemicellulases.

10. The process of claim 8, wherein the pretreating causes hemicellulose and cellulose in the complex carbohydrates to become more accessible to hydrolysis.

11. The process of claim 8, wherein the ammonia treatment process is an ammonia fiber expansion process.

12. The process of claim 10, wherein the pretreating further comprises performing an additional pretreatment process selected from a dilute-acid treatment process, a dilute-base treatment process, an ammonia recycle percolation process, a steam explosion process, a high-temperature exposure process, an irradiation process, a lime pretreatment process, an alkaline wet oxidation process, and an ionic liquid pretreatment process and combinations thereof.

13. The process of claim 8, wherein the process further comprises:

drying the substantially solid portion to form distillers dried grains with solubles (DDGS).

14. The process of claim 8, further comprising fermenting the sugars.

15. The process of claim 14, wherein the hydrolyzing of the complex carbohydrates in the pretreated lignocellulosic material and the fermenting of the sugars are performed in a single vessel.

16. A process for producing sugars from corn stover comprising:

pretreating the corn stover to produce pretreated corn stover, wherein the pretreating is performed with an ammonia treatment process which causes hemicellulose and cellulose in the corn stover to become more accessible to hydrolysis, wherein ammonia in the ammonia treatment process is present in a concentration ranging from about 0.2 to about 4 grams ammonia per gram of the lignocellulosic material;

adding thin corn grain stillage to an aqueous medium containing the pretreated corn stover, wherein the thin corn grain stillage comprises crude corn protein and the aqueous medium comprises about 0.5 wt. % to about 99 wt. % thin corn grain stillage relative to the aqueous medium; and

hydrolyzing the hemicellulose and cellulose in the aqueous medium with one or more hydrolyzing enzymes to form the sugars.

17. The process of claim 16, wherein the pretreated corn stover comprises glucan and the aqueous medium has a glucan loading as a component of pretreated corn stover ranging from about 1 wt. % to about 4 wt. %.

18. The process of claim 16, wherein the pretreated corn stover comprises glucan, and the aqueous medium has a glucan loading as a component of pretreated corn stover ranging from about 4 wt. % to about 15 wt. %.

19. The process of claim 16, further comprising fermenting the sugars.

20. A process for producing sugars from a lignocellulosic material and corn comprising:

pretreating the lignocellulosic material with an ammonia treatment process to produce a pretreated lignocellulosic material, wherein ammonia in the ammonia pretreatment process is present in a concentration ranging from about 0.2 to about 4 grams ammonia per gram of the lignocellulosic material;

adding corn grain stillage to the pretreated lignocellulosic material, wherein the corn grain stillage comprises crude corn protein; and

in an aqueous medium, hydrolyzing complex carbohydrates in the pretreated lignocellulosic material with one or more hydrolyzing enzymes to form sugars, wherein the aqueous medium contains the pretreated lignocellulosic material, the corn grain stillage, and the one or more hydrolyzing enzymes, further wherein the aqueous medium comprises about 0.5 wt. % to about 99 wt. % corn grain stillage relative to the aqueous medium.

21. The method of claim 20 wherein the corn grain stillage comprises at least 0.1% corn grain protein.

22. The method of claim 20 wherein the corn grain stillage comprises about 0.1% to about 10% corn grain protein.