Methods, devices, kits and compositions for detecting whipworm

• US 8,367,808 B2
• Filed: 07/14/2011
• Issued: 02/05/2013
• Est. Priority Date: 05/19/2008
• Status: Active Grant

• Current Assignee: Monsanto Company (Bayer AG), IDEXX Laboratories Incorporated
• Original Assignee: Divergence, Inc. (Bayer AG), IDEXX Laboratories Incorporated
• Inventors: Elsemore, David Allen, Flynn, Laurie A., Crawford, Michael
• Primary Examiner(s): BASKAR, PADMAVATHI
• Application Number:
  US13/182,585
• Publication Number:
  US 20120208983A1
• Time in Patent Office: 572 Days
• Field of Search: None
• US Class Current: 530/388.6
• CPC Class Codes: C07K 14/43536 from worms
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: G01N 2333/43526 from worms
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Abstract

Methods, devices, kits and compositions for detecting the presence or absence of whipworm in a fecal sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of whipworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, roundworm, and heartworm. Confirmation of the presence or absence of whipworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

Claims

1. An isolated antibody wherein the antibody specifically binds to the polypeptide consisting of the amino acid sequence of SEQ ID NO:

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, or SEQ ID NO;

9.

2. The isolated antibody of claim 1, wherein the antibody is obtained by immunization with a polypeptide consisting of the amino acid sequence of SEQ ID NO:

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, or SEQ ID NO;

9.

3. The isolated antibody of claim 1, wherein the antibody is detectably labeled.

4. The isolated antibody of claim 1, wherein the antibody specifically binds to whipworm antigen in a fecal sample obtained from a whipworm-infected mammal.

5. The isolated antibody of claim 4, wherein the whipworm infected mammal is further infected with one or more of hookworm, roundworm and heartworm and the antibody does not bind to any antigen from the one or more of hookworm, roundworm, or heartworm that may be present in the fecal sample.

6. The isolated antibody of claim 1, wherein the antibody is immobilized on a solid support.

7. The isolated antibody of claim 4, wherein the whipworm is Trichuris vulpis.

8. The isolated antibody of claim 5, wherein the hookworm is Ancylostoma caninum, the roundworm is Toxocara canis, and/or the heartworm is Dirofilaria immitis.

9. The isolated antibody of claim 1, wherein the antibody is a single chain antibody, or an antigen binding fragment of an antibody.

10. The isolated antibody of claim 1, wherein the antibody is a polyclonal or monoclonal antibody.