Substrate for culture of cardiovascular tissue

• US 8,372,433 B2
• Filed: 01/18/2008
• Issued: 02/12/2013
• Est. Priority Date: 01/18/2007
• Status: Active Grant

• Current Assignee: Gunze Limited
• Original Assignee: Gunze Limited
• Inventors: Shinoka, Toshiharu, Matsumura, Goki, Ikada, Yoshito, Matsuda, Shojiro, Sakamoto, Yuki, Taira, Tsuguyoshi
• Primary Examiner(s): Ford, Allison
• Application Number:
  US12/523,124
• Publication Number:
  US 20100055781A1
• Time in Patent Office: 1,852 Days
• Field of Search: None
• US Class Current: 424/484
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/48 with macromolecular fillers
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: A61L 27/58 Materials at least partially resorbable by the body
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

The scaffold for culturing a cardiovascular tissue of the present invention is a scaffold for culturing a cardiovascular tissue, which is tubular, and comprises a foamed polymer comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material, the foamed polymer comprising lactide (D, L, DL isomer)-ε-caprolactone copolymer containing lactide (D, L, DL isomer) in a content of 50 to 54 mole % and ε-caprolactone in a content of 50 to 46 mole %, and the reinforcing material being covered with the foamed polymer.

Claims

1. A scaffold for culturing a cardiovascular tissue,

which is tubular, and comprises a foamed polymer comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material,

the reinforcing material comprising a twisted yarn comprising a twisted bioabsorbable multifilament yarn,

the reinforcing material being situated at a center or on an outer plane of the scaffold, and

the foamed polymer being situated on an inner plane of the scaffold.

2. A method of producing a cardiovascular tissue for transplantation comprising a scaffold having a surface covered with a cell,

which comprises seeding a cell in vitro on the scaffold for culturing a cardiovascular tissue according to claim 1, and further culturing the cell in vitro.

3. A method of regenerating a cardiovascular tissue,

which comprises seeding a cell in vitro on the scaffold for culturing a cardiovascular tissue according to claim 1, and further culturing the cell to regenerate a cardiovascular tissue in vitro.

4. A cardiovascular tissue for transplantation,

which is obtained by seeding an endothelial cell, a marrow cell, a smooth muscle cell or a fibroblast in vitro on the scaffold for culturing a cardiovascular tissue according to claim 1, and further culturing the cell in vitro.

5. A scaffold for culturing a cardiovascular tissue,

which is tubular, and comprises a foamed polymer comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material, and a reinforcing yarn comprising a bioabsorbable material,

the reinforcing yarn and the reinforcing material being situated at a center or on an outer plane of the foamed polymer,

the foamed polymer being situated on an inner plane of the scaffold, and

the reinforcing yarn being wound in a spiral form, a ring form, or a X-shaped form

wherein the reinforcing yarn is a monofilament yarn having a cross-sectional diameter of 0.1 mm to 1 mm.

6. The scaffold for culturing a cardiovascular tissue according to claim 5,

wherein the reinforcing yarn comprises at least one kind of compound selected from the group consisting of poly-L-lactide, a lactide ε

-caprolactone copolymer and a glycolic acid-ε

-caprolactone copolymer.

7. A method of producing a cardiovascular tissue for transplantation comprising a scaffold having a surface covered with a cell,

which comprises seeding a cell in vitro on the scaffold for culturing a cardiovascular tissue according to claim 5, and further culturing the cell in vitro.

8. A method of regenerating a cardiovascular tissue,

which comprises seeding a cell in vitro on the scaffold for culturing a cardiovascular tissue according to claim 5, and further culturing the cell to regenerate a cardiovascular tissue in vitro.

9. A cardiovascular tissue for transplantation,

which is obtained by seeding an endothelial cell, a marrow cell, a smooth muscle cell or a fibroblast in vitro on the scaffold for culturing a cardiovascular tissue according to claim 5, and further culturing the cell in vitro.