Method for extracellular matrix mediated differentiation and proliferation of stem cells
First Claim
1. A method of directing differentiation of a human stem cell into a pre-selected cell type, said method comprising culturing the human stem cell on extracellular matrix extracted from differentiated support cells and in a stem cell culture medium comprising from about 20% (v/v) to about 70% (v/v) medium conditioned by the differentiated support cells and free from the differentiated support cells, to promote differentiation of the stem cell into said pre-selected cell type, wherein (i) the human stem cell is a human embryonic stem cell, the pre-selected cell type is a proximal tubule cell, and the differentiated support cells are proximal tubule cells;
- (ii) the human stem cell is a human embryonic stem cell or a human mesenchymal stem cell, the pre-selected cell type is an insulin-producing cell, and the differentiated support cells are pancreatic insulin-secreting cells;
or (iii) the human stem cell is a human mesenchymal stem cell, the pre-selected cell type is an osteoblast, and the differentiated support cells are osteoblasts, and wherein for (ii) the stem cell culture medium comprises 10% serum.
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Abstract
There is provided a method of culturing a stem cell on extracellular matrix extracted from support cells and in a stem cell culture medium comprising medium conditioned by the support cells.
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10 Claims
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1. A method of directing differentiation of a human stem cell into a pre-selected cell type, said method comprising culturing the human stem cell on extracellular matrix extracted from differentiated support cells and in a stem cell culture medium comprising from about 20% (v/v) to about 70% (v/v) medium conditioned by the differentiated support cells and free from the differentiated support cells, to promote differentiation of the stem cell into said pre-selected cell type, wherein (i) the human stem cell is a human embryonic stem cell, the pre-selected cell type is a proximal tubule cell, and the differentiated support cells are proximal tubule cells;
- (ii) the human stem cell is a human embryonic stem cell or a human mesenchymal stem cell, the pre-selected cell type is an insulin-producing cell, and the differentiated support cells are pancreatic insulin-secreting cells;
or (iii) the human stem cell is a human mesenchymal stem cell, the pre-selected cell type is an osteoblast, and the differentiated support cells are osteoblasts, and wherein for (ii) the stem cell culture medium comprises 10% serum. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- (ii) the human stem cell is a human embryonic stem cell or a human mesenchymal stem cell, the pre-selected cell type is an insulin-producing cell, and the differentiated support cells are pancreatic insulin-secreting cells;
Specification