Method and apparatus for identifying proteins in mixtures

• US 8,373,115 B2
• Filed: 04/30/2012
• Issued: 02/12/2013
• Est. Priority Date: 05/20/2004
• Status: Active Grant

• Current Assignee: Waters Technologies Corporation (Waters Corporation)
• Original Assignee: Waters Technologies Corporation (Waters Corporation)
• Inventors: Geromanos, Scott J., Gorenstein, Marc Victor, Silva, Jeffrey Cruz, Li, Guo-Zhong
• Primary Examiner(s): Vanore, David A
• Application Number:
  US13/460,128
• Publication Number:
  US 20120267522A1
• Time in Patent Office: 288 Days
• Field of Search: 250/281, 250/282, 250/284, 250/288
• US Class Current: 250/282
• CPC Class Codes: G01N 2030/8831 involving peptides or proteins
• CPC Class Codes: G01N 30/7233 interfaced to liquid or supercritical fluid chromatograph interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04
• CPC Class Codes: G01N 33/6848 Methods of protein analysis involving mass spectrometry
• CPC Class Codes: G16B 15/00 ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
• CPC Class Codes: H01J 49/0036 Step by step routines describing the handling of the data generated during a measurement
• CPC Class Codes: H01J 49/0431 for liquid samples interfaces to liquid chromatographs G01N30/7233
• CPC Class Codes: H01J 49/426 Methods for controlling ions

Abstract

Protein identification in a complex sample begins by selecting a database having proteins likely to be in the sample. In-silico digestion is performed and a target peptide is selected from produced peptides. The masses of the Y- and B-ion fragments of the target peptide are determined. These masses are used to search previously obtained low- and high-energy AMRTs obtained from LC/MS analysis of the complex sample for masses on the list. Any mass observed in the data within a detection threshold are considered a hit. If enough hits accumulate in a given retention time, the target peptide is identified as being in the sample. The list of peptides identified in the complex sample can be used to identify the proteins present in the sample, track the chromatographic retention times of peptides between samples, and quantitate the peptides and proteins present in complex samples.

Claims

1. A method for identifying proteins in a mixture comprising:

performing an LC/MS experiment using the mixture and acquiring first mass information and first retention time information associated with precursors and second mass information and second retention time information associated with fragments of the precursors;

selecting a target peptide, wherein said target peptide is a theoretical peptide having a target precursor and corresponding Y and B fragment ions of the target precursor, wherein the target precursor and said corresponding Y and B fragment ions of the target precursor have associated masses;

comparing the associated masses of the target precursor and the corresponding Y and B fragment ions of the target precursor to the first mass information and the second mass information;

detecting mass matches based on said comparing;

determining retention times of the mass matches; and

identifying, in accordance with said mass matches and the retention times of the mass matches, whether the target peptide is present in the mixture.

2. The method of claim 1, wherein the first mass information and the first retention time information are obtained as a result of applying a low energy mode in a mass spectrometer and the second mass information and the second retention time information are obtained as a result of applying an elevated energy mode in a mass spectrometer to fragment the precursors.

3. The method of claim 2, wherein the low energy mode is applied by applying a low voltage in a collision cell of the mass spectrometer and the elevated energy mode is applied by applying a high voltage in the collision cell.

4. The method of claim 2, further comprising switching between the low energy mode and the elevated energy mode in accordance with a protocol that alternates application of the low energy mode and the elevated energy mode with a sufficient frequency whereby each of the low energy mode and the elevated energy mode is applied a plurality of times during a chromatographic peak width.

5. The method of claim 1, further comprising creating a detection chromatogram.

6. The method of claim 5, wherein the detection chromatogram is a histogram including a series of bins, wherein each of the bins has a center corresponding to a retention time and each of the bins has a width corresponding to a retention time interval, said histogram identifying a number of mass matches in each of the bins whereby each mass match of one of the bins has a retention time occurring in said one bin.

7. The method of claim 5, wherein the detection chromatogram is derived using Gaussian shaped peaks.

8. The method of claim 1, wherein a mass match is determined if any of said target precursor and said corresponding Y and B fragment ions of the target precursor have a corresponding mass in any of the first mass information and the second mass information.

9. The method of claim 1, wherein the target peptide is selected from a database and the mass matches are determined in accordance with a search tolerance.

10. The method of claim 9, wherein the search tolerance is automatically determined from data using statistics, or is user-specified.

11. The method of claim 1, further comprising:

determining a detection threshold; and

identifying whether a peptide is present based on whether a count of mass matches in a particular retention time interval exceeds the detection threshold.

12. The method of claim 11, further comprising:

estimating a noise floor associated with the detection threshold; and

analyzing a significance of a peptide identification based on the count and the estimate of the noise floor.

13. The method of claim 11, wherein said detection threshold varies with a complexity of the mixture.

14. A system comprising:

a liquid chromatograph to which a mixture is applied to separate the mixture producing a separated mixture;

a mass spectrometer that measures masses in the separated mixture;

a database from which a target peptide is selected;

a computer coupled to the database and an output of the mass spectrometer, said computer having code executing thereon which, when executed, causes the computer to perform a method, the method comprising;

acquiring first mass information and first retention time information associated with precursors and second mass information and second retention time information associated with fragments of the precursors;

selecting said target peptide, wherein said target peptide is a theoretical peptide having a target precursor and corresponding Y and B fragment ions of the target precursor, wherein the target precursor and said corresponding Y and B fragment ions of the target precursor have associated masses;

comparing the associated masses of the target precursor and the corresponding Y and B fragment ions of the target precursor to the first mass information and the second mass information;

detecting mass matches based on said comparing;

determining retention times of the mass matches; and

identifying, in accordance with said mass matches and the retention times of the mass matches, whether the target peptide is present in the mixture.

15. The system of claim 14, wherein the first mass information and the first retention time information are obtained as a result of applying a low energy mode in the mass spectrometer and the second mass information and the second retention time information are obtained as a result of applying an elevated energy mode in a mass spectrometer to fragment the precursors.

16. The system of claim 15, wherein the low energy mode is applied by applying a low voltage in a collision cell of the mass spectrometer and the elevated energy mode is applied by applying a high voltage in the collision cell.

17. The system of claim 15, wherein the mass spectrometer is switched between the low energy mode and the elevated energy mode in accordance with a protocol that alternates application of the low energy mode and the elevated energy mode with a sufficient frequency whereby each of the low energy mode and the elevated energy mode is applied a plurality of times during a chromatographic peak width.

18. The system of claim 14, wherein a detection chromatogram is created.

19. The system of claim 18, wherein the detection chromatogram identifies a number of ions within each of a plurality of retention time intervals.

20. The system of claim 18, wherein the detection chromatogram is derived using Gaussian shaped peaks.

21. A non-transitory computer readable medium comprising code stored thereon for identifying proteins in a mixture, the non-transitory computer readable medium comprising code stored thereon for:

performing an LC/MS experiment using the mixture and acquiring first mass information and first retention time information associated with precursors and second mass information and second retention time information associated with fragments of the precursors;

selecting a target peptide, wherein said target peptide is a theoretical peptide having a target precursor and corresponding Y and B fragment ions of the target precursor, wherein the target precursor and said corresponding Y and B fragment ions of the target precursor have associated masses;

comparing the associated masses of the target precursor and the corresponding Y and B fragment ions of the target precursor to the first mass information and the second mass information;

detecting mass matches based on said comparing;

determining retention times of the mass matches; and

identifying, in accordance with said mass matches and the retention times of the mass matches, whether the target peptide is present in the mixture.

22. A non-transitory computer readable medium comprising code stored thereon for identifying proteins in a mixture, the non-transitory computer readable medium comprising code stored thereon for:

receiving a first set of mass and retention time information associated with precursors and a second set of mass and retention time information associated with fragments of the precursors, wherein said first set of mass and retention time information and said second set of mass and retention time information are obtained from an LC/MS experiment using the mixture;

selecting a target peptide;

determining, for the target peptide, a target precursor and corresponding Y and B fragment ions of the target precursor, wherein the target precursor and said corresponding Y and B fragment ions of the target precursor have associated masses;

determining mass matches for a substantially same retention time in said first set of mass and retention time information and said second set of mass and retention time information in accordance with the masses of said target precursor and said corresponding Y and B fragment ions of the target precursor; and

identifying, in accordance with said mass matches for a substantially same retention time, whether the target peptide is present in the mixture.