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Methods and compositions related to determination and use of white blood cell counts

  • US 8,377,398 B2
  • Filed: 05/31/2006
  • Issued: 02/19/2013
  • Est. Priority Date: 05/31/2005
  • Status: Expired due to Fees
First Claim
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1. A cartridge for differential assay of white blood cell populations, the cartridge comprising:

  • a chamber;

    a pad connected to the chamber via a fluid channel, wherein the pad is a reagent pad containing therein three binding agents each labeled with a different fluorophore;

    a microsieve positioned at least partially within the chamber, wherein pores of the microsieve are configured to retain white blood cells from a blood sample and to allow red blood cells to pass through the microsieve,wherein the fluorophore-labeled binding agents are flowed from the pad and over the microsieve and allowed to differentially bind to populations of white blood cells previously captured on the microsieve,wherein an image can be obtained from the microsieve, andwherein the fluorophore-labeled binding agents specifically bind to different populations of white blood cells so as to allow the different populations of cells to be distinguishably detectable by fluorescence.

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