Test strip and method for measuring blood cholesterol level using the same

• US 8,377,684 B2
• Filed: 03/11/2010
• Issued: 02/19/2013
• Est. Priority Date: 06/04/2009
• Status: Active Grant

• Current Assignee: Osang Healthcare Co., Ltd.
• Original Assignee: INFOPIA CO., LTD
• Inventors: Bae, Byeong-Woo, Lee, Sung-Dong, Kho, Byung-Hoon, Ryu, Ji-Eon, Kim, Jin-Kyeong, Joung, Hyou-Arm
• Primary Examiner(s): Hanley, Susan
• Assistant Examiner(s): Shen, Bin
• Application Number:
  US12/722,147
• Publication Number:
  US 20100311091A1
• Time in Patent Office: 1,076 Days
• Field of Search: 435/287.1, 435/287.7
• US Class Current: 435/287.1
• CPC Class Codes: C12Q 1/60 involving cholesterol
• CPC Class Codes: G01N 33/526 the element being adapted for a specific analyte
• CPC Class Codes: G01N 33/92 involving lipids, e.g. cholesterol , lipoproteins, or their receptors steroid hormones G01N33/743

Abstract

Disclosed are a test strip and a method for measuring medical data. The test strip comprises an upper cover having one or more application holes; a lower support having one or more detected parts at positions corresponding to detecting units of the measuring apparatus; an erythrocyte and LDL cholesterol-filtering layer designed to filter off both of erythrocytes and LDL cholesterol from an applied blood sample by agglutinating erythrocytes and precipitating LDL cholesterol; and a reaction layer in which the blood sample free of erythrocytes and LDL cholesterol is reacted with an reagent, the filtering layer and the reaction layer being stacked between the lower support and the upper cover. Structured to filter off erythrocytes and low-density lipoprotein (LDL) cholesterol from a blood sample in a single layer, the test strip of the present invention can be made to be slim and allows a low volume of blood to be analyzed.

Claims

1. A test strip for measuring medical data in association with a measuring apparatus, comprising:

an upper cover having one or more application holes for receiving a blood sample;

a filtering layer having at least first filtering region and second filtering region, the first filtering region for filtering off erythrocytes from the blood sample by agglutinating erythrocytes and the second filtering region for filtering off both of erythrocytes and low-density lipoprotein (LDL) cholesterol from the blood sample by agglutinating erythrocytes and precipitating LDL cholesterol; and

a reaction layer having first reaction region and second reaction region, wherein the filtered blood sample is reacted with an reagent in the first reaction region and the second reaction region,

wherein the first filtering region and the second filtering region are disposed in a substantially same plane adjacent to each other, wherein the first reaction region is communicated with the first filtering region and the second reaction region is communicated with the second filtering region.

2. The test strip of claim 1, wherein the second filtering region includes a hematogglutinin selected from a group consisting of lectin, a cationic polymer, a saccharide and a combination thereof.

3. The test strip of claim 1, wherein the second filtering region includes phosphotungstic acid (PTA) as an LDL cholesterol-precipitating agent.

4. The test strip of claim 1, wherein the second filtering region includes dextran sulfate as a low-density lipoprotein (LDL) cholesterol-precipitating agent.

5. The test strip of claim 1, wherein the second filtering region includes as a low-density lipoprotein (LDL) cholesterol-precipitating agent, a combination of a divalent cation and one selected from a group consisting of phosphotungstic acid (PTA), dextran sulfate and a combination thereof.