Lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same
First Claim
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1. An assay device comprising:
- a porous, hydrophilic medium in the form of a substantially planar sheet,a pattern of fluid-impervious barriers inserting through the thickness of the sheet thereby to define boundaries of plural assay regions each comprising a channel region defining a flow path within the sheet along which fluids pass in a direction parallel to the plane of the sheet by capillary action, andone or more assay reagents disposed in fluid communication with respective assay regions which alone or together provide a visible color change or intensity in the device of a clinically relevant concentration of an analyte, which visible color change or intensity can be digitized to reproduce an image of said visual color change or intensity which also is clinically relevant.
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Abstract
Embodiments of the invention provide lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same. Under one aspect, an assay device includes a porous, hydrophilic medium; a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium; and an assay reagent in the assay region.
118 Citations
15 Claims
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1. An assay device comprising:
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a porous, hydrophilic medium in the form of a substantially planar sheet, a pattern of fluid-impervious barriers inserting through the thickness of the sheet thereby to define boundaries of plural assay regions each comprising a channel region defining a flow path within the sheet along which fluids pass in a direction parallel to the plane of the sheet by capillary action, and one or more assay reagents disposed in fluid communication with respective assay regions which alone or together provide a visible color change or intensity in the device of a clinically relevant concentration of an analyte, which visible color change or intensity can be digitized to reproduce an image of said visual color change or intensity which also is clinically relevant. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification