Methods of measuring concentration of component in biochemical sample and estimating reliability of test result
First Claim
1. A method of measuring a concentration of a component in a biochemical sample, the method comprising:
- injecting a plurality of standard samples into a plurality of chambers of a first centrifugal microfluidic device, wherein the plurality of standard samples includes at least one common component, and each of the plurality of standard samples has a different concentration of the component;
measuring absorbances of the component in each of the plurality of standard samples contained in the chambers of the centrifugal microfluidic device at a plurality of different wavelength bands by emitting from a plurality of light sources a plurality of light beams having the plurality of wavelength bands to the plurality of standard samples and sensing by a plurality of optical detectors intensities of the plurality of light beams that have passed through the plurality of standard samples;
determining a regression equation of a relationship between the concentration of the component and the measured absorbances of the component in the plurality of standard samples at the plurality of wavelength bands; and
measuring absorbances of the biochemical sample contained in a plurality of chambers of a second centrifugal microfluidic device at the plurality of wavelength bands by emitting from the plurality of light sources the plurality of light beams having the plurality of wavelength bands to the biochemical sample and sensing by the plurality of optical detectors intensities of the plurality of light beams that have passed through the biochemical sample and applying the measured absorbances of the biochemical sample to the regression equation to calculate the concentration of the component in the biochemical sample.
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Abstract
Provided are a method of measuring a concentration of a component in a biochemical sample such as serum, and a method of estimating the reliability of a test result using the method. The method of measuring a concentration of a component includes: preparing plural standard samples having at least one common component which has varying concentrations; measuring absorbances of the component in the standard samples at each of the concentrations for plural light beams having different wavelength bands; determining a regression equation of a relationship between the concentration of the component and the measured absorbances at plural wavelength bands; and applying the regression equation by measuring absorbances at the plural wavelength bands of the biochemical sample and applying the measured plural absorbances of the biochemical sample to the regression equation to calculate the concentration of the component.
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Citations
16 Claims
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1. A method of measuring a concentration of a component in a biochemical sample, the method comprising:
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injecting a plurality of standard samples into a plurality of chambers of a first centrifugal microfluidic device, wherein the plurality of standard samples includes at least one common component, and each of the plurality of standard samples has a different concentration of the component; measuring absorbances of the component in each of the plurality of standard samples contained in the chambers of the centrifugal microfluidic device at a plurality of different wavelength bands by emitting from a plurality of light sources a plurality of light beams having the plurality of wavelength bands to the plurality of standard samples and sensing by a plurality of optical detectors intensities of the plurality of light beams that have passed through the plurality of standard samples; determining a regression equation of a relationship between the concentration of the component and the measured absorbances of the component in the plurality of standard samples at the plurality of wavelength bands; and measuring absorbances of the biochemical sample contained in a plurality of chambers of a second centrifugal microfluidic device at the plurality of wavelength bands by emitting from the plurality of light sources the plurality of light beams having the plurality of wavelength bands to the biochemical sample and sensing by the plurality of optical detectors intensities of the plurality of light beams that have passed through the biochemical sample and applying the measured absorbances of the biochemical sample to the regression equation to calculate the concentration of the component in the biochemical sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of measuring a concentration of a component in a biochemical sample, the method comprising:
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injecting n standard samples having at least one common component into a plurality of chambers of a centrifugal microfluidic device, wherein each of the n standard samples has a different concentration of the component and n is a natural number; measuring m×
n absorbances of the component in the n standard samples contained in the chambers of the centrifugal microfluidic device at m wavelength bands by emitting from a plurality of light sources m light beams each having a different wavelength band of the m wavelength bands to the n standard samples and sensing by a plurality of optical detectors intensities of the m light beams that have passed through the n standard samples;determining a regression equation of the concentration of the component, in which the absorbances at the m wavelength bands are included as independent variables, based on the measured m×
n absorbances; andmeasuring absorbances of the biochemical sample contained in a plurality of chambers of a second centrifugal microfluidic device at the m wavelength bands by emitting from the plurality of light sources the m light beams having the m wavelength bands to the biochemical sample and sensing by the plurality of optical detectors intensities of the plurality of light beams that have passed through the biochemical sample and applying the m absorbances of the biochemical sample to the corresponding independent variables of the regression equation to calculate the concentration of the component in the biochemical sample. - View Dependent Claims (16)
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Specification