Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes

• US 8,380,457 B2
• Filed: 08/31/2010
• Issued: 02/19/2013
• Est. Priority Date: 08/29/2007
• Status: Active Grant

• Current Assignee: Canon USA, Inc. (Canon Inc.)
• Original Assignee: Canon USA, Inc. (Canon Inc.)
• Inventors: Coursey, Johnathan S.
• Primary Examiner(s): Lau, Tung S
• Application Number:
  US12/872,046
• Publication Number:
  US 20110056926A1
• Time in Patent Office: 903 Days
• Field of Search: 702/133
• US Class Current: 702/133
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 7/00 Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices
• CPC Class Codes: B01L 7/525 with physical movement of samples between temperature zones
• CPC Class Codes: G01R 27/02 Measuring real or complex resistance, reactance, impedance, or other two-pole characteristics derived therefrom, e.g. time constant by measuring phase angle only G01R25/00

Abstract

The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature of each heater electrode by determining the resistance of each heater electrode.

Claims

1. A method for determining the temperature of each of a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit sharing a common lead connecting the electrodes to a power supply, said method comprising the steps of:

a. for each heater electrode in said plurality of multiplexed heater electrodes;

(1) connecting the power supply to a set of two or more other heater electrodes, and

(2) measuring a voltage drop at said heater electrode, wherein the voltage drop is based on the equivalent resistance of a parallel combination of the set of said other heater electrodes in series with said heater electrode;

b. computing a resistance of each of the plurality of multiplexed heater electrodes by solving for the resistance of each heater electrode based at least in part on the measured voltage drops; and

c. deriving the temperature using a processor of each of the plurality of multiplexed heater electrodes from the computed resistance of each electrode.

2. The method of claim 1, wherein:

each set of two or more of the other heater electrodes includes the same number of other heater electrodes;

each set of two or more of the other heater electrodes is distinct; and

each heater electrode is included in the same number of sets of two or more of the other heater electrodes.

3. The method of claim 1, wherein computing the resistance of each of the plurality of multiplexed heater electrodes comprises providing an initial estimate of the resistance of each heater electrode and iteratively refining the estimates based at least in part on the measured resistances until the successive refinements are below a predetermined threshold.

4. The method of claim 1, wherein the step of connecting the power supply to a set of two or more of the other heater electrodes comprises actuating digital switches to effectuate the connections.

5. The method of claim 1, further comprising:

providing a pulse width modulated power source to each of the heater electrodes;

wherein the steps of connecting the power supply to a set of two or more of the other heater electrodes, and of measuring a voltage drop at the heater electrode occur during the off time of the pulse width modulated power source.