Genetic test module with low oligonucleotide probe mass and reagent volumes
First Claim
1. A test module for performing a genetic diagnostic assay, the test module comprising:
- an outer casing dimensioned for hand-held portability, the outer casing having a receptacle for a biological sample containing target nucleic acid sequences;
an array of chambers containing probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, each of the chambers containing less than 270 picograms of probe;
a flow-path extending from the receptacle to the probes; and
,a reagent reservoir containing a reagent for addition to the sample in the flow-path upstream of the probes, the reagent reservoir having a volume less than 1000,000,000 cubic microns;
wherein,the reagent reservoir has an outlet with a surface tension valve for retaining the reagent in the reservoir with a reagent meniscus until contact with the biological sample removes the reagent meniscus.
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Abstract
A test module for performing a genetic diagnostic assay, the test module having an outer casing dimensioned for hand-held portability, the outer casing having a receptacle for a biological sample containing target nucleic acid sequences, an array of chambers containing probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, a flow-path extending from the inlet to the probes, and, a reagent reservoir containing a reagent for addition to the sample in the flow-path upstream of the probes, wherein, each of the chambers contains less than 270 picograms of probe and the reagent reservoir has a volume less than 1000,000,000 cubic microns.
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Citations
19 Claims
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1. A test module for performing a genetic diagnostic assay, the test module comprising:
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an outer casing dimensioned for hand-held portability, the outer casing having a receptacle for a biological sample containing target nucleic acid sequences; an array of chambers containing probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, each of the chambers containing less than 270 picograms of probe; a flow-path extending from the receptacle to the probes; and
,a reagent reservoir containing a reagent for addition to the sample in the flow-path upstream of the probes, the reagent reservoir having a volume less than 1000,000,000 cubic microns;
wherein,the reagent reservoir has an outlet with a surface tension valve for retaining the reagent in the reservoir with a reagent meniscus until contact with the biological sample removes the reagent meniscus. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification