Method for determining analyte concentration in biological fluid using electrochemical sensor
First Claim
1. A method of determining the concentration of an analyte in a biological fluid, the method comprising:
- contacting an electrochemical sensor with the biological fluid, wherein the electrochemical sensor comprises;
a working electrode;
a counter electrode; and
a crosslinked polymer matrix disposed on at least a portion of the working electrode, the crosslinked polymer matrix comprising;
polyaniline or a ring substituted polyaniline and poly(2-acrylamido-2-methyl-propane sulfonic acid) (PAAMSA);
a water soluble crosslinker; and
one or more enzymes, wherein at least one of the enzymes is a redox enzyme; and
determining the concentration of the analyte in the biological fluid.
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Abstract
A polymer matrix that may coated on an electrode is created by co-crosslinking (1) an adduct of a polyaniline formed by templated oxidative polymerization on a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme. The polymer matrix may be hydrated, and the absorbed water may make it permeable to, for example, glucose. The polyaniline may be polyaniline itself or a substituted polyaniline; the water-soluble crosslinker may be poly(ethylene glycol)diglycidyl ether, and the redox enzyme may be glucose oxidase. The polymer matrix may be produced by co-crosslinking (1) an adduct of an electrically conductive polymer and a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme in a single step at an about neutral pH, curing by drying. After hydration, the crosslinked polymer matrix may form a 3-dimensional glucose-permeable bioelectrocatalyst, catalyzing the electrooxidation of glucose.
16 Citations
12 Claims
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1. A method of determining the concentration of an analyte in a biological fluid, the method comprising:
contacting an electrochemical sensor with the biological fluid, wherein the electrochemical sensor comprises; a working electrode; a counter electrode; and a crosslinked polymer matrix disposed on at least a portion of the working electrode, the crosslinked polymer matrix comprising; polyaniline or a ring substituted polyaniline and poly(2-acrylamido-2-methyl-propane sulfonic acid) (PAAMSA); a water soluble crosslinker; and one or more enzymes, wherein at least one of the enzymes is a redox enzyme; and
determining the concentration of the analyte in the biological fluid.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
Specification