Cell culture apparatus, cell culture method, cell culture program and cell culture system

• US 8,383,395 B2
• Filed: 11/01/2006
• Issued: 02/26/2013
• Est. Priority Date: 11/01/2005
• Status: Expired due to Fees

• Current Assignee: Medinet Co. Ltd.
• Original Assignee: Medinet Co. Ltd.
• Inventors: Hata, Norihiko, Jinguji, Hidemasa, Noguchi, Atsutaka, Sato, Shiho, Sato, Kazutoshi, Ariyoshi, Naoko, Hosoi, Akihiro
• Primary Examiner(s): Beisner, William H
• Assistant Examiner(s): HENKEL, DANIELLE B
• Application Number:
  US12/084,363
• Publication Number:
  US 20090042293A1
• Time in Patent Office: 2,309 Days
• Field of Search: 435/286.1, 435/289.1, 435/383, 435/288.7, 382/133
• US Class Current: 435/286.1
• CPC Class Codes: C12M 23/58 Reaction vessels connected in series or in parallel combinations of bioreactors with other apparatus, C12M43/00
• CPC Class Codes: C12M 41/14 Incubators; Climatic chambers per se B01L1/00
• CPC Class Codes: C12M 41/46 of cellular or enzymatic activity or functionality, e.g. cell viability
• CPC Class Codes: C12M 41/48 Automatic or computerized control automatic analysis G01N35/00

Abstract

The invention intends to provide a cell culture apparatus which is able to realize an adequate culture according to the culture state of cells while alleviating the labor of an operator. The cell culture apparatus includes a culture bag for causing the cells to proliferate, a cell inoculation cassette (or culture bag as an antibody stimulating and proliferation culture vessel) for stimulating the cells by an inducer for the proliferation, a culture medium cassette for storing a culture medium supplied to the culture bag and the cell inoculation cassette, a CCD camera 88 for acquiring images of the cells in the cell inoculation cassette, and an image processing computer and an operation control computer for determining the culture state (proliferation capability and proliferation ability of the cells) of the cells from the images of the cells acquired by the CCD camera, and causing a culture operation to be carried out on the basis of the determination.

Claims

1. A cell culture apparatus comprising:

a culture vessel which cultures cells;

a culture medium storage unit which stores a culture medium supplied to the culture vessel;

a platform on which the culture vessel is placed, wherein the platform includes a non-elevating part which is a portion of the platform on which the culture vessel is placed, and at least one area-changing part which is another portion of the platform on which the culture vessel is placed, wherein the at least one area-changing part is movable upward and downward such that the non-elevating part of the platform and the at least one area-changing part of the platform are arrangeable on different planes, and wherein a culture area of the culture vessel is changed by moving the at least one area-changing part;

an image acquiring unit which acquires images of the cells in the culture vessel; and

a control unit which determines a culture state of the cells from the images of the cells acquired by the image acquiring unit and controls a culture operation to be carried out based on the determination,

wherein the control unit controls the platform to change the culture area of the culture vessel.

2. The cell culture apparatus according to claim 1, wherein the culture vessel includes a proliferation culture vessel for causing the cells to proliferate and a function expressing culture vessel for causing the cells to express the functions, and the image acquiring unit acquires the images of the cells in the function expressing culture vessel.

3. The cell culture apparatus according to claim 2, wherein the function expressing culture vessel is an inducer stimulating culture vessel for stimulating cells by an inducer for the proliferation, and the control unit determines the proliferation capability of the cells and the proliferation ability of the cells on the basis of the images of the cells in the inducer stimulating culture vessel and controls timing of transferring the cells from the inducer stimulating culture vessel to the proliferation culture vessel and the culture operation, wherein the culture operation includes supply of the culture medium from the culture medium storage unit to the proliferation culture vessel.

4. The cell culture apparatus according to claim 2, wherein the function expressing culture vessel is a differentiation-inducing culture vessel for causing the cells to differentiate, and the control unit controls a differentiation-inducing operation based on the images of the cells in the differentiation-inducing culture vessel.

5. The cell culture apparatus according to claim 1, wherein the culture medium storage unit comprises a cassette structure and is connected to the culture vessel.

6. The cell culture apparatus according to claim 2, wherein the function expressing culture vessel comprises a cassette structure, and is connected to the proliferation culture vessel and the culture medium storage unit.

7. The cell culture apparatus according to claim 2, wherein the culture medium storage unit, the function expressing culture vessel and the proliferation culture vessel make up a closed system.

8. The cell culture apparatus according to claim 2, wherein a liquid reservoir is selectively formed in the proliferation culture vessel, and the cell and the culture medium are stored in the liquid reservoir in an initial stage of culture in the proliferation culture vessel.

9. The cell culture apparatus according to claim 2, wherein the control unit causes cells proliferated in the proliferation culture vessel to be introduced into the function expressing culture vessel, and the image acquiring unit acquires images of the introduced cells.

10. The cell culture apparatus according to claim 2, wherein the culture medium storage unit includes a used culture medium storage vessel which is adapted to store a used culture medium installed therein together with a culture medium storage vessel which is able to store a culture medium to be supplied to the culture vessel, and the used culture medium in the proliferation culture vessel is discharged to the used culture medium storage vessel and is stored therein.

11. The cell culture apparatus according to claim 2, wherein the culture medium storage unit is adapted to mount a cell collecting vessel for collecting the cells mounted thereto, and the cells condensed in the proliferation culture vessel are collected in the cell collecting vessel.