Detection and quantification of biomolecules using mass spectrometry
First Claim
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1. A detector oligonucleotide comprising:
- a complementary portion that is complementary to a target nucleic acid, which complementary portion comprises a non-cleavable nucleotide incorporated at its 5′
end, anda contiguous non-complementary portion linked to the 5′
end of the complementary portion, that is susceptible to cleavage by a cleavage agent, which cleavage can produce a mass distinguishable product (MDP).
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Abstract
The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.
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Citations
15 Claims
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1. A detector oligonucleotide comprising:
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a complementary portion that is complementary to a target nucleic acid, which complementary portion comprises a non-cleavable nucleotide incorporated at its 5′
end, anda contiguous non-complementary portion linked to the 5′
end of the complementary portion, that is susceptible to cleavage by a cleavage agent, which cleavage can produce a mass distinguishable product (MDP). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification