Formation of immobilized biological layers for sensing

• US 8,389,042 B2
• Filed: 05/23/2012
• Issued: 03/05/2013
• Est. Priority Date: 12/20/2007
• Status: Expired due to Fees

• Current Assignee: Abbott Point Of Care Incorporated (Abbott Laboratories)
• Original Assignee: Abbott Point Of Care Incorporated (Abbott Laboratories)
• Inventors: Collier, Gordon Bruce, Macleod, Jason Andrew, Wong, Anjulia, Nemeth, Attila Csaba
• Primary Examiner(s): LEE, DORIS L
• Application Number:
  US13/478,260
• Publication Number:
  US 20120270756A1
• Time in Patent Office: 286 Days
• Field of Search: 427/2.13
• US Class Current: 427/2.13
• CPC Class Codes: B05C 11/1034 specially designed for conducting intermittent application of small quantities, e.g. drops, of coating material B05C5/0225, B05C11/1026 take precedence
• CPC Class Codes: B05C 5/0291 the material being discharged on the work through discrete orifices as discrete droplets, beads or strips that coalesce on the work or are spread on the work so as to form a continuous coating
• CPC Class Codes: B05C 9/12 the auxiliary operation being performed after the application B05C9/14 takes precedence
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 1/42 involving phosphatase
• CPC Class Codes: C12Q 1/58 involving urea or urease

Abstract

The invention is directed to enzyme immobilization compositions comprising: one or more enzymes, a humectant, an acrylic-based monomer, a water-soluble organic photo-initiator and a water-soluble acrylic-based cross-linker in a substantially homogeneous aqueous mixture. The invention is also directed to methods for forming sensors comprising such compositions and to apparati for forming arrays of immobilized layers on an array of sensors by dispensing such compositions onto a substrate.

Claims

1. A method of forming an immobilized layer on a sensor on a substantially planar surface comprising the steps of:

(a) dispensing a controlled volume of a photoformable matrix at a pre-selected location on said surface, wherein the photoformable matrix comprises a biologically active material selected from the group consisting of protein, enzyme, antibody, antibody fragment, RNA, single stranded DNA and double stranded DNA, an acrylic-based monomer, a water soluble organic photo-initiator and a water soluble acrylic-based cross-linker in an aqueous mixture; and

(b) applying a UV radiation beam onto an area substantially covering said pre-selected location, starting at a predetermined time after said volume has been dispensed and for a predetermined duration at a predetermined intensity, to form said immobilized layer.

2. The method claim 1, wherein the immobilized layer is an enzyme layer.

3. A method of forming an array of immobilized layers on an array of sensors on a substantially planar surface comprising the steps of:

(a) dispensing a sequence of controlled volumes of a photoformable matrix at a pre-selected set of locations on said surface, and

(b) applying a UV radiation beam onto an area substantially covering each said pre-selected location, in sequence, starting at a predetermined time after each controlled volume has been dispensed, and applying said radiation at a predetermined intensity for a predetermined duration, to form said immobilized array of layer wherein the UV radiation bean is applied to the Nth minus X pre-selected location while dispensing occurs at the Nth pre-selected location, where X is equal to an integer from 1 to 10.

4. The method of claim 3, wherein the predetermined time is in the range of about 0.1 to about 10 seconds.

5. The method of claim 3, wherein the predetermined duration is in the range of about 0.1 to about 10 seconds.

6. The method of claim 3, wherein said UV radiation is in the wavelength range of about 185 to 400 nm.

7. The method of claim 3, wherein said UV radiation intensity is in the range of about 100 to 400 mW/cm².

8. The method of claim 3, wherein said planar surface is a silicon wafer and the pre-selected set of locations is an array of sensors on said wafer.