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Detection of nucleic acids by type-specific hybrid capture method

  • US 8,389,219 B2
  • Filed: 11/19/2009
  • Issued: 03/05/2013
  • Est. Priority Date: 06/15/2000
  • Status: Expired due to Fees
First Claim
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1. A method of detecting a target nucleic acid consisting essentially of:

  • a) hybridizing a single-stranded or partially single-stranded target nucleic acid to a capture sequence probe and a signal sequence probe, wherein the capture sequence probe and the signal sequence probe hybridize to non-overlapping regions within the target nucleic acid and not to each other, wherein said hybridization forms an RNA/DNA hybrid between said signal sequence probe and the target nucleic acid; and

    b) adding a blocker probe to the hybridization reaction, wherein said blocker probe hybridizes to excess non-hybridized capture sequence probes; and

    c) detecting the RNA/DNA hybrid by binding an antibody that recognizes the RNA/DNA hybrid to said hybrid, wherein said antibody is detectably labeled.

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